Nat Prod Sci.  2018 Jun;24(2):82-87. 10.20307/nps.2018.24.2.82.

Determination of Silybin B in the Different Parts of Silybum marianum using HPLC-UV

• Rodriguez
• Quilantang NG
• Lee JS
• Lee JM
• Kim HY
• Shim JS
• Lee S

• KMID: 2416066
• DOI: http://doi.org/10.20307/nps.2018.24.2.82

Abstract

Silymarin is the standardized extract from Silybum marianum which consists mainly of flavonoids and polyphenols. It is highly regarded for its hepatoprotective ability. Silybin B is a flavonolignan and one of the active components of silymarin. The content of silybin B in various parts of S. marianum was analyzed by HPLC-UV. Results show that the extract of seeds contain the highest amount of silybin B (7.434 mg/g DW). The petioles of S. marianum showed a low content of silybin B. This study revealed that seeds of S. marianum contain high amount of silybin B and could be a good source of the compound.

Keyword

HPLC-UV; Silybum marianum; silybin B; silymarin

MeSH Terms

Flavonoids
Milk Thistle*
Polyphenols
Silymarin
Flavonoids
Polyphenols
Silymarin

Figure

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  Fig. 1. Structure of silybin B.

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  Fig. 2. HPLC chromatograms of silybin B (A) and the EtOH extracts of leaves (B), petioles (C), flowers (D), stems (E), seeds (F), and germinated seeds (G) of S. marianum.

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  Fig. 3. HPLC chromatograms of silybin B (A) and the EtOH extracts of seeds (B) of S. marianum

Reference

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