Endocrinol Metab.  2017 Mar;32(1):18-22. 10.3803/EnM.2017.32.1.18.

A Simple Outline of Methods for Protein Isolation and Purification

• Lee CH

• KMID: 2413281
• DOI: http://doi.org/10.3803/EnM.2017.32.1.18

Abstract

At the summer workshop of the Korean Endocrine Society held in 2016, some examples of protein experiments were discussed in the session entitled "All about working with proteins." In contrast to what the title suggested, it was unrealistic to comprehensively discuss all protein analytical methods. Therefore, the goal was to outline protein experimental techniques that are useful in research or in bench work. In conversations with clinicians, however, I have always felt that researchers who do not engage in bench science have different demands than those who do. Protein research tools that are useful in bench science may not be very useful or effective in the diagnostic field. In this paper, I provide a general summary of the protein analytical methods that are used in basic scientific research, and describe how they can be applied in the diagnostic field.

Keyword

Chromatography; Molecular imaging; Immunoenzyme techniques

MeSH Terms

Chromatography
Education
Immunoenzyme Techniques
Molecular Imaging

Reference

1. Burgess RR, Deutscher MP. Methods in enzymology: guide to protein purification. 2nd ed. Vol. 436. San Diego: Academic Press;2009.

2. Janson JC. Protein purification: principles, high resolution methods, and applications. Hoboken: John Wiley & Sons;2011.

3. Wang Y, Fan Z, Shao L, Kong X, Hou X, Tian D, et al. Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications. Int J Nanomedicine. 2016; 11:3287–3303.

4. Konning D, Zielonka S, Grzeschik J, Empting M, Valldorf B, Krah S, et al. Camelid and shark single domain antibodies: structural features and therapeutic potential. Curr Opin Struct Biol. 2016; 45:10–16.

5. Ozalp VC, Kavruk M, Dilek O, Bayrac AT. Aptamers: molecular tools for medical diagnosis. Curr Top Med Chem. 2015; 15:1125–1137.

6. Schwamborn K, Kriegsmann M, Weichert W. MALDI imaging mass spectrometry: from bench to bedside. Biochim Biophys Acta. 2016; 10. 31. [Epub]. DOI: 10.1016/j.bbapap.2016.10.014.

7. Petras D, Jarmusch AK, Dorrestein PC. From single cells to our planet-recent advances in using mass spectrometry for spatially resolved metabolomics. Curr Opin Chem Biol. 2017; 36:24–31.