Tissue Eng Regen Med.  2017 Dec;14(6):755-762. 10.1007/s13770-017-0070-1.

Novel Approach Toward the Generation of Tissue Engineered Heart Valve by Using Combination of Antioxidant and Detergent: A Potential Therapy in Cardiovascular Tissue Engineering

• Tardalkar
• Desai S
• Adnaik A
• Bohara R
• Joshi M

• KMID: 2411869
• DOI: http://doi.org/10.1007/s13770-017-0070-1

Abstract

To develop decellularized heart valve scaffold from porcine for heart valve regeneration. Porcine heart valves were decellularized with unique optimized approach by using 1% sodium dodecyl sulfate solution and 5% dimethyl sulfoxide for the first time. Effect of decellularization process on scaffold were characterized by hematoxylin-eosin, 4"²,6-diamidino-2-phenylindole, Masson's trichrome, alcian blue staining and scanning electron microscopy for extracellular matrix (ECM) analysis in scaffold. The results showed that developed protocol for decellularization of heart valve scaffold shown complete removal of all cellular components, without changing the properties of ECM. The developed protocol was successfully used for heart valve ECM scaffolds development from porcine. The developed protocol seems to be promising solution for the heart valve tissue engineering application.

Keyword

Decellularization; Heart valve; Tissue engineering; Scaffold; Cardiovascular

MeSH Terms

Alcian Blue
Detergents*
Dimethyl Sulfoxide
Extracellular Matrix
Heart Valves*
Heart*
Microscopy, Electron, Scanning
Regeneration
Sodium Dodecyl Sulfate
Tissue Engineering*
Alcian Blue
Detergents
Dimethyl Sulfoxide
Sodium Dodecyl Sulfate