Korean J Intern Med.
2018 Mar;33(2):313-322. 10.3904/kjim.2016.140.
Correlation between NADPH oxidase-mediated oxidative stress and dysfunction of endothelial progenitor cell in hyperlipidemic patients
- Affiliations
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- 1Department of Laboratory Medicine, Xiangya School of Medicine, Changsha, China. xjluo22@csu.edu.cn
- 2Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha, China.
- 3Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, China.
- 4Hunan Provincial Key Laboratory of Cardiovascular Research, School of Pharmaceutical Sciences, Central South University, Changsha, China.
- KMID: 2411543
- DOI: http://doi.org/10.3904/kjim.2016.140
Abstract
- BACKGROUND/AIMS
NADPH (nicotinamide adenine dinucleotide phosphate) oxidase (NOX)-mediated oxidative stress plays a key role in promotion of oxidative injury in the cardiovascular system. The aim of this study is to evaluate the status of NOX in endothelial progenitor cells (EPCs) of hyperlipidemic patients and to assess the correlation between NOX activity and the functions EPCs.
METHODS
A total of 30 hyperlipidemic patients were enrolled for this study and 30 age-matched volunteers with normal level of plasma lipids served as controls. After the circulating EPCs were isolated, the EPC functions (migration, adhesion and tube formation) were evaluated and the status of NOX (expression and activity) was examined.
RESULTS
Compared to the controls, hyperlipidemic patients showed an increase in plasma lipids and a reduction in EPC functions including the attenuated abilities in adhesion, migration and tube formation, concomitant with an increase in NOX expression (NOX2 and NOX4), NOX activity, and reactive oxygen species production. The data analysis showed negative correlations between NOX activity and EPC functions.
CONCLUSIONS
There is a positive correlation between the NOX-mediated oxidative stress and the dysfunctions of circulating EPCs in hyperlipidemic patients, and suppression of NOX might offer a novel strategy to improve EPCs functions in hyperlipidemia.
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