Korean J Intern Med.  2017 Sep;32(5):883-889. 10.3904/kjim.2015.254.

Caveolin-1 is involved in high glucose accelerated human glomerular mesangial cell senescence

• Feng X
• Gao W
• Li Y

• KMID: 2411491
• DOI: http://doi.org/10.3904/kjim.2015.254

Abstract

BACKGROUND/AIMS
: We demonstrated the role of caveolin-1 involved in high glucose (HG)-induced glomerular mesangial cells (GMCs) senescence.
METHODS
: HG was used to stimulate GMCs. The telomere lengths were analyzed by Southern blot. β-Galactosidase staining was determined. The expressions of caveolin-1 and P53 proteins were determined by Western blot.
RESULTS
: Treatment with high concentrations of glucose induced GMC senescence accompanied by shortened telomere length and increase of β-galactosidase staining as well as P53 protein, which was abrogated after application of caveolin-1-siRNA.
CONCLUSIONS
: This study proved that HG induced cell senescence in GMCs. The caveolin-1 is involved in HG-induced mesangial cell senescence, and blocking caveolin-1 significantly reduced cell senescence. The effect of caveolin-1 is mediated by P53 pathway.

Keyword

Mesangial cells; Aging; Caveolin-1

MeSH Terms

Aging*
Blotting, Southern
Blotting, Western
Caveolin 1*
Cell Aging
Glucose*
Humans*
Mesangial Cells*
Telomere
Caveolin 1
Glucose