Investig Clin Urol.  2017 Jul;58(4):289-295. 10.4111/icu.2017.58.4.289.

The renoprotective effects of mannitol and udenafil in renal ischemia-reperfusion injury model

• Özlülerden Y
• ToktaÅŸ C
• Aybek H
• Küçükatay V
• Åžen Türk N
• Zumrutbas

• KMID: 2410297
• DOI: http://doi.org/10.4111/icu.2017.58.4.289

Abstract

PURPOSE
The aim of this study was to investigate and compare the effects of udenafil and mannitol in an experimental renal ischemia-reperfusion (I/R) injury model.
MATERIALS AND METHODS
A total of 64 female Wister Albino rats were used. Right nephrectomy was performed in all groups. In the control group; I/R injury was not performed. In the I/R group; left renal pedicle was clamped for 45 minutes and then underwent 60 minutes and 24 hours of reperfusion. In the mannitol group; 1 mL 20% mannitol was given intravenously 15 minutes before clamping. In the udenafil group; 10-mg/kg udenafil was given orally 1 hour before clamping. Creatinine (Cr), blood urea nitrogen (BUN), Cr clearance, malondialdehyde, neutrophil gelatinase associated lipocalin (NGAL), histological examination and DNA damage (Comet Assay method) levels were compared in tissue, serum and urine samples.
RESULTS
Udenafil had a better protective effect than mannitol according to biochemical parameters (Cr, BUN, Cr clearance, and NGAL levels) and histopathological findings when compared with the I/R group. In the Comet sampling analysis no significant difference was detected.
CONCLUSIONS
Udenafil has a better renoprotective effect than mannitol against I/R injury and this effect supports more functional improvements. Further clinical trials are needed to demonstrate those effects and clinical utility of udenafil for that purpose in humans.

Keyword

Comet assay; Ischemia reperfusion injury; Mannitol; Ngal protein; Udenafil

MeSH Terms

Animals
Blood Urea Nitrogen
Comet Assay
Constriction
Creatinine
DNA Damage
Female
Gelatinases
Humans
Lipocalins
Malondialdehyde
Mannitol*
Nephrectomy
Neutrophils
Rats
Reperfusion
Reperfusion Injury*
Creatinine
Gelatinases
Lipocalins
Malondialdehyde
Mannitol

Figure

• 

  Fig. 1 Creatinine and creatinine clearance levels of the control and the study groups. I/R, ischemia-reperfusion; M, mannitol; U, udenafil. *Statiscally significant (p<0.01) when U and M groups compared to I/R group.

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