Nat Prod Sci.  2018 Mar;24(1):13-20. 10.20307/nps.2018.24.1.13.

Estragole Exhibits Anti-inflammatory Activity with the Regulation of NF-κB and Nrf-2 Signaling Pathways in LPS-induced RAW 264.7 cells

Affiliations
  • 1Department of Food and Life Science, Pukyong National University, Busan 608-737, Republic of Korea. choijs@pknu.ac.kr
  • 2Department of Pharmaceutical Engineering, Sangji University, Wonju 220-702, Republic of Korea.
  • 3Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju 561-756, Republic of Korea. jungha@jbnu.ac.kr

Abstract

Estragole is a naturally occurring phenylpropanoid obtained from essential oils found in a broad diversity of plants. Although the phenylpropanoids show many biological activities, clear regulation of the inflammatory signaling pathways has not yet been determined. Here, we scrutinized the anti-inflammatory effect of estragole. The anti-inflammatory effect of estragole was determined through the inhibitory mechanisms of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), nuclear factor kappa B (NF-κB), and mitogen-activated protein kinases (MAPK) pathways and the activation of nuclear factor erythroid 2-related factor 2 (Nrf-2)/heme oxygenase (HO)-1 pathways in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Estragole significantly inhibited NO production, iNOS and COX-2 expression as well as LPS-induced NF-κB and MAPK activation. Furthermore, estragole suppressed LPS-induced intracellular ROS production but up-regulated the stress response gene HO-1 via the activation of transcription factor Nrf-2. These findings demonstrate that estragole inhibits the LPS-induced expression of inflammatory mediators via the down-regulation of iNOS, COX-2, NF-κB, and MAPK pathways, as well as the up-regulation of the Nrf-2/HO-1 pathway, indicating that this phenylpropanoid has potential therapeutic and preventive applications in various inflammatory diseases.

Keyword

Essential oils; Estragole; Anti-inflammation; Nrf-2; HO-1; RAW 264.7 cells

MeSH Terms

Down-Regulation
Mitogen-Activated Protein Kinases
NF-kappa B
Nitric Oxide Synthase Type II
Oils, Volatile
Prostaglandin-Endoperoxide Synthases
RAW 264.7 Cells*
Transcription Factors
Up-Regulation
Mitogen-Activated Protein Kinases
NF-kappa B
Nitric Oxide Synthase Type II
Oils, Volatile
Prostaglandin-Endoperoxide Synthases
Transcription Factors
Full Text Links
  • NPS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error