Mycobiology.  2009 Jun;37(2):114-120.

Isolation, Identification and Optimal Culture Conditions of Streptomyces albidoflavus C247 Producing Antifungal Agents against Rhizoctonia solani AG2-2

• Islam MR
• Jeong YT
• Ryu YJ
• Song CH
• Lee YS

Abstract

Streptomyces albidoflavus C247 was isolated from the soil of the Gyeongsan golf course in Korea. Physiological, biochemical and 16S rDNA gene sequence analysis strongly suggested that the isolate belonged to Streptomyces albidoflavus. Preliminary screening revealed that the isolate was active against fungi and bacteria. Self-directing optimization was employed to determine the best combination of parameters such as carbon and nitrogen source, pH and temperature. Nutritional and culture conditions for the production of antibiotics by this organism under shake-flask conditions were also optimized. Maltose (5%) and soytone (5%) were found to be the best carbon and nitrogen sources for the production of antibiotics by S. albidoflavus C247. Additionally, 62.89% mycelial growth inhibition was achieved when the organism was cultured at 30degrees C and pH 6.5. Ethyl acetate (EtOAc) was the best extraction solvent for the isolation of the antibiotics, and 100 microg/ml of EtOAc extract was found to inhibit 60.27% of the mycelial growth of Rhizoctonia solani AG2-2(IV) when the poison plate diffusion method was conducted.

Keyword

Antifungal agent; Rhizoctonia solani AG2-2(IV); Streptomyces albidoflavus C247

MeSH Terms

Acetates
Anti-Bacterial Agents
Antifungal Agents
Bacteria
Carbon
DNA, Ribosomal
Fungi
Hydrogen-Ion Concentration
Korea
Maltose
Nitrogen
Rhizoctonia
Sequence Analysis
Soil
Streptomyces
Acetates
Anti-Bacterial Agents
Antifungal Agents
Carbon
DNA, Ribosomal
Maltose
Nitrogen
Soil