Mycobiology.  2012 Mar;40(1):47-52.

Antitumor and Immunostimulating Activities of Elfvingia applanata Hot Water Extract on Sarcoma 180 Tumor-bearing ICR Mice

• Shim SM
• Lee JS
• Lee TS
• Lee UY

Abstract

Elfvingia applanata, a medicinal mushroom belonging to Basidiomycota, has been used in the effort to cure cancers of the esophagus and stomach, and is also known to have inhibitory effects on hepatitis B virus infection. The hot water soluble fraction (as Fr. HW) was extracted from fruiting bodies of the mushroom. In vitro cytotoxicity tests showed that hot water extract was not cytotoxic against cancer cell lines such as Sarcoma 180, HT-29, HepG2, and TR at concentrations of 10~2,000 microg/mL. Intraperitoneal injection with Fr. HW resulted in a life prolongation effect of 45.2% in mice previously inoculated with Sarcoma 180. Treatment of Fr. HW resulted in a 2.53-fold increase in the numbers of murine spleen cells at a concentration of 50 microg/mL, compared with control. Incubation of murine spleen cells with Fr. HW at a concentration of 500 microg/mL resulted in improved immune-potwntiating activity of B lymphocytes through an 8.3-folds increase in alkaline phosphatase activity, compared with control. Fr. HW generated 12.5 microM of nitric oxide (NO) when cultured with RAW 264.7, a mouse macrophage cell line, at the concentration of 50 microg/mL, while lipopolysaccharide, a positive control, produced 15.2 microM of NO. Therefore, the results suggested that antitumor activities of Fr. HW from E. applanata might, in part, be due to host mediated immunostimulating activity.

Keyword

Anticancer activity; Elfvingia applanata; Hot water extract; Immunostimulating

MeSH Terms

Agaricales
Alkaline Phosphatase
Animals
B-Lymphocytes
Basidiomycota
Cell Line
Esophagus
Fruit
Hepatitis B virus
Injections, Intraperitoneal
Life Support Care
Macrophages
Mice
Mice, Inbred ICR
Nitric Oxide
Sarcoma
Sarcoma 180
Spleen
Stomach
Water
Alkaline Phosphatase
Nitric Oxide
Water