Restor Dent Endod.  2018 Feb;43(1):e11. 10.5395/rde.2018.43.e11.

Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopy

• Küçükkaya Eren S
• UzunoÄŸlu
• Sezer B
• Yılmaz Z
• Boyacı IH

• KMID: 2406096
• DOI: http://doi.org/10.5395/rde.2018.43.e11

Abstract

OBJECTIVES
This study aimed to introduce the use of laser-induced breakdown spectroscopy (LIBS) for evaluation of the mineral content of root canal dentin, and to assess whether a correlation exists between LIBS and scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS) methods by comparing the effects of irrigation solutions on the mineral content change of root canal dentin.
MATERIALS AND METHODS
Forty teeth with a single root canal were decoronated and longitudinally sectioned to expose the canals. The root halves were divided into 4 groups (n = 10) according to the solution applied: group NaOCl, 5.25% sodium hypochlorite (NaOCl) for 1 hour; group EDTA, 17% ethylenediaminetetraacetic acid (EDTA) for 2 minutes; group NaOCl+EDTA, 5.25% NaOCl for 1 hour and 17% EDTA for 2 minutes; a control group. Each root half belonging to the same root was evaluated for mineral content with either LIBS or SEM/EDS methods. The data were analyzed statistically.
RESULTS
In groups NaOCl and NaOCl+EDTA, the calcium (Ca)/phosphorus (P) ratio decreased while the sodium (Na) level increased compared with the other groups (p < 0.05). The magnesium (Mg) level changes were not significant among the groups. A significant positive correlation was found between the results of LIBS and SEM/EDS analyses (r = 0.84, p < 0.001).
CONCLUSIONS
Treatment with NaOCl for 1 hour altered the mineral content of dentin, while EDTA application for 2 minutes had no effect on the elemental composition. The LIBS method proved to be reliable while providing data for the elemental composition of root canal dentin.

Keyword

Dentin; Endodontics; Microscopy; Spectrum analysis

MeSH Terms

Calcium
Dental Pulp Cavity*
Dentin*
Edetic Acid
Endodontics
Magnesium
Methods
Microscopy
Miners*
Sodium
Sodium Hypochlorite
Spectrum Analysis*
Tooth
Calcium
Edetic Acid
Magnesium
Sodium
Sodium Hypochlorite

Figure

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  Figure 1. Schematic diagram of experimental setup for the laser-induced breakdown spectroscopy (LIBS) system. BNC, Bayonet Neill–Concelman; SMA, shape memory alloy.

• 

  Figure 2. Laser-induced breakdown spectroscopy (LIBS) spectra for the groups. Ca, calcium; P, phosphorus; Mg, magnesium; Na, sodium; NaOCl, sodium hypochlorite; EDTA, ethylenediaminetetraacetic acid.

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  Figure 3. The change in the elemental distribution of the experimental groups compared with the control group. (A) Laser-induced breakdown spectroscopy (LIBS) analysis, (B) scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS) analysis. Ca, calcium; P, phosphorus; Mg, magnesium; Na, sodium; NaOCl, sodium hypochlorite; EDTA, ethylenediaminetetraacetic acid.

Reference

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