Med Lasers.  2024 Dec;13(4):195-202. 10.25289/ML.24.030.

Optimizing experimental conditions of femtosecond laser-induced breakdown spectroscopy for differentiating melanoma from the normal dermis in Republic of Korea: an experimental study

• Choi J

• KMID: 2564468
• DOI: http://doi.org/10.25289/ML.24.030

Abstract

Background
Femtosecond laser-induced breakdown spectroscopy (fs-LIBS) is a powerful tool for biomedical diagnostics because it provides real-time, in situ elemental analysis with high spatial resolution. This study used fs-LIBS to distinguish melanoma from normal dermis tissue.
Methods
A crater size of approximately 20 μm and an enhanced signal-to-background ratio of critical emission lines were achieved by optimizing the key experimental parameters, such as laser energy, gate delay, and spectral normalization.
Results
Principal component analysis and support vector machine algorithms were applied to the normalized spectra, resulting in 96.4% classification accuracy.
Conclusion
fs-LIBS, under the appropriate experimental conditions and data processing, provides precise tissue analysis and has significant potential for advancing bio-imaging and early cancer detection.

Keyword

Laser-induced breakdown spectroscopy; Spectrum analysis; Melanoma; Classification; Support vector machine