Restor Dent Endod.  2018 Feb;43(1):e1. 10.5395/rde.2018.43.e1.

Root canal irrigants influence the hydrophobicity and adherence of Staphylococcus epidermidis to root canal dentin: an in vitro study

• Nagendrababu V
• Sheriff Sultan O
• Kannathasan S
• Patel AS
• Chitra E
• Neelakantan P
• Davamani F

• KMID: 2406086
• DOI: http://doi.org/10.5395/rde.2018.43.e1

Abstract

OBJECTIVES
To determine the effect of root canal irrigants on the hydrophobicity and adherence of Staphylococcus epidermidis (S. epidermidis) to root canal dentin in vitro.
MATERIALS AND METHODS
Root dentin blocks (n = 60) were randomly divided into 4 groups based on the irrigation regimen: group 1, saline; group 2, 5.25% sodium hypochlorite (NaOCl); group 3, 5.25% NaOCl followed by 17% ethylenediaminetetraacetic acid (EDTA); group 4, same as group 3 followed by 2% chlorhexidine (CHX). The hydrophobicity of S. epidermidis to root dentin was calculated by cell surface hydrophobicity while the adherence was observed by fluorescence microscopy, and bacteria were quantified using ImageJ software (National Institutes of Health). Statistical analysis of the data was done using Kruskal-Wallis test and Mann-Whitney U test (p = 0.05).
RESULTS
The hydrophobicity and adherence of S. epidermidis to dentin were significantly increased after irrigating with group 3 (NaOCl-EDTA) (p < 0.05), whereas in group 4 (NaOCl-EDTA-CHX) both hydrophobicity and adherence were significantly reduced (p < 0.05).
CONCLUSIONS
The adherence of S. epidermidis to dentin was influenced differently by root canal irrigants. Final irrigation with CHX reduces the bacterial adherence and may impact biofilm formation.

Keyword

Adherence; Chlorhexidine; Ethylenediaminetetraacetic acid; Hydrophobicity; Sodium hypochlorite; Staphylococcus epidermidis

MeSH Terms

Academies and Institutes
Bacteria
Biofilms
Chlorhexidine
Dental Pulp Cavity*
Dentin*
Edetic Acid
Hydrophobic and Hydrophilic Interactions*
In Vitro Techniques*
Microscopy, Fluorescence
Root Canal Irrigants*
Sodium Hypochlorite
Staphylococcus epidermidis*
Staphylococcus*
Chlorhexidine
Edetic Acid
Root Canal Irrigants
Sodium Hypochlorite