J Korean Acad Conserv Dent.  2007 Mar;32(2):121-129. 10.5395/JKACD.2007.32.2.121.

Evaluation of time-dependent antimicrobial effect of sodium dichloroisocyanurate (NaDCC) on Enterococcus faecalis in the root canal

• Kim HJ
• Park SH
• Cho KM
• Kim JW

• KMID: 2175845
• DOI: http://doi.org/10.5395/JKACD.2007.32.2.121

Abstract

The purpose of this study was to assess the antibacterial effect of sodium dichloroisocyanurate (NaDCC), sodium hypochlorite (NaOCl), and chlorhexidine (CHX) on Enterococcus faecalis and to evaluate and to compare the time-dependant antimicrobial effect of NaDCC with NaOCl and CHX in the root canal in vitro before and after instrumentation. Extracted human single teeth were prepared by serial instrumentation technique. The samples were autoclaved and contaminated for 3 days with E. faecalis monocultures. The teeth were then divided into 4 groups. Each group was irrigated and inserted with 2% NaOCl, 2% NaDCC, 2% CHX and sterilized saline. After 6, 12, 24, 72h, and 1 week incubation, sterilized paper point was inserted into the root canal. Paper points containing root canal contents were then placed on the agar plate. And then each root canal was prepared with #4 and #5 GG (Gates-Glidden) drill. The debris were collected in the sterilized microtube and the plates were incubated at 37degrees C in an increased CO2 atmosphere. After 24h incubation the growth of bacteria around the paper points were measured. NaOCl and NaDCC solution shows similar antimicrobial effect for E. faecalis at 6, 12, 24, 72h and 1 week. In control group, irrigated with sterilized saline, no antimicrobial effect was observed. The results are in agreement with other investigators, who have shown the bactericidal property and possibility of NaDCC as a root canal irrigation solution. Thus it seems that NaDCC solutions can be clinically applied into the root canal within 1 week after dilution.

Keyword

Sodium dichloroisocyanurate; Sodium hypochlorite; Chlorhexidine; E. faecalis; Root canal irrigants; Antimicrobial effect

MeSH Terms

Agar
Atmosphere
Bacteria
Chlorhexidine
Dental Pulp Cavity*
Enterococcus faecalis*
Enterococcus*
Humans
Research Personnel
Root Canal Irrigants
Sodium Hypochlorite
Sodium*
Tooth
Agar
Chlorhexidine
Root Canal Irrigants
Sodium
Sodium Hypochlorite

Figure

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  Figure 1 Molecular structure of Sodium dichloroisocyanurate

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  Figure 2 Schematic illustration of incubating tooth block

• 

  Figure 3 Schematic illustration of sampling of dentin.

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