J Biomed Transl Res.  2017 Dec;18(4):130-135. 10.12729/jbtr.2017.18.4.130.

Reactive oxygen species (ROS) induces distinct gene expression in human fibroblast like synoviocytes (HFLS)

• Kil KM
• Jung SH
• Jeon IS
• Cho BK
• Park JK
• Park KJ
• Kim YM
• Choi JK

• KMID: 2405356
• DOI: http://doi.org/10.12729/jbtr.2017.18.4.130

Abstract

Adhesive capsulitis of the shoulder is a common cause of pain that occurs during shoulder movement, thereby restricting shoulder rotation in clinical practice. Although most patients respond to pain relief treatment (NSAID or corticosteroids) by improving their range of motion, it remains poorly understood without any definitive treatment algorithm. In addition to immune cells, synoviocytes, chondrocytes and osteoblasts in the joint are known to produce pro-inflammatory mediators such as reactive oxygen species (ROS), inflammatory cytokines and lipid mediators, presumably contributing to the pathogenesis of osteoarthritis (OA) and adhesive capsulitis. Although inflammation and also fibrosis are proposed to be the basic pathological changes of a frozen shoulder, there is a lack of information regarding the downstream targets of the pro-inflammatory ROS signaling pathway in the synoviocytes and also how these ROS targets are modulated at the transcription level by a corticosteroid - dexamethasone. In this study, we used human fibroblast like synoviocytes (HFLS) to characterize the signaling targets of ROS by employing a human DNA microarray tool and studied the role of dexamethasone in this process. Our data suggest that several genes such as FOS, FOSB and NFkBIZ, which are known to be involved in pro- or anti- inflammation response, are modulated at the transcription level by ROS and dexamethasone.

Keyword

synoviocytes; reactive oxygen species; dexamethasone; adhesive capsulitis; DNA microarray

MeSH Terms

Bursitis
Chondrocytes
Cytokines
Dexamethasone
Fibroblasts*
Fibrosis
Gene Expression*
Humans*
Inflammation
Joints
Oligonucleotide Array Sequence Analysis
Osteoarthritis
Osteoblasts
Range of Motion, Articular
Reactive Oxygen Species*
Shoulder
Cytokines
Dexamethasone
Reactive Oxygen Species