Int J Oral Biol.  2017 Dec;42(4):149-153. 10.11620/IJOB.2017.42.4.149.

Induction of Prostaglandin Eâ‚‚ by Porphyromonas gingivalis in Human Dental Pulp Cells

• Kim SH
• Paek YW
• Kang IC

• KMID: 2404838
• DOI: http://doi.org/10.11620/IJOB.2017.42.4.149

Abstract

Cyclooxygenase-2 (COX-2)-mediated prostaglandin E₂ (PGE₂) plays a key role in development and progression of inflammatory responses and Porphyromonas gingivalis is a common endodontic pathogen. In this study, we investigated induction of COX-2 and PGE₂ by P. gingivalis in human dental pulp cells (HDPCs). P. gingivalis increased expression of COX-2, but not that of COX-1. Increased levels of PGE₂ were released from P. gingivalis-infected HDPCs and this PGE₂ increase was blocked by celecoxib, a selective COX-2 inhibitor. P. gingivalis activated all three types of mitogen-activated protein kinases (MAPKs). P. gingivalis-induced activation of nuclear factor-κB (NF-κB) was demonstrated by the results of phosphorylation of NF-κ B p65 and degradation of inhibitor of κB-α (IκB-α). Pharmacological inhibition of each of the three types of MAPKs and NF-κB substantially attenuated P. gingivalis induced PGE2 production. These results suggest that P. gingivalis should promote endodontic inflammation by stimulating dental pulp cells to produce PGE₂.

Keyword

Human dental pulp cells; prostaglandin Eâ‚‚; cyclooxygenase-2; Porphyromonas gingivalis

MeSH Terms

Celecoxib
Cyclooxygenase 2
Dental Pulp*
Dinoprostone
Humans*
Mitogen-Activated Protein Kinases
Phosphorylation
Porphyromonas gingivalis*
Porphyromonas*
Pulpitis
Celecoxib
Cyclooxygenase 2
Dinoprostone
Mitogen-Activated Protein Kinases