Nat Prod Sci.  2017 Dec;23(4):281-285. 10.20307/nps.2017.23.4.281.

Anti-tumor Activity of Saussurea laniceps against Pancreas Adenocarcinoma

• Lee KH
• Kim BS
• Rhee KH

• KMID: 2401581
• DOI: http://doi.org/10.20307/nps.2017.23.4.281

Abstract

The purpose of this study was to confirm the anti-tumor activity of an ethanol extract of Saussurea laniceps against pancreatic tumor and to isolate the active compound from S.laniceps extract. Treatment with S.laniceps extract and hispidulin inhibited proliferation of pancreatic cell lines, such as Capan-1, Capan-2, Panc-1 and S2-013 in a dose-dependent manner using the hollow fiber assay. Hispidulin showed typical hallmarks of apoptotic cell death a significant anti-tumor activity on Capan-2 cells at a dose of 100 mg/kg and 200 mg/kg. S.laniceps has potential cytotoxic and apoptotic effects on human pancreatic carcinoma cells. Its mechanism of action might be associated with the apoptotic cell death through DNA fragmentation.

Keyword

Saussurea laniceps; Pancreatic carcinoma; Hispidulin; Hollow fiber assay

MeSH Terms

Adenocarcinoma*
Cell Death
Cell Line
DNA Fragmentation
Ethanol
Humans
Pancreas*
Saussurea*
Ethanol

Figure

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  Fig. 1. Structure of hispidulin.

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  Fig. 2. Extirpated hollow fibers from animals.

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  Fig. 3. Effect of Hispidulin on DNA fragmentation of Capan-2 cells. (A) Ladder marker; (B) Control; (C) 2 µg/mL of Hispidulin; (D) 10 µg/mL of Hispidulin; (E) 2 µg/mL of Gemicitabine.

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