Exp Mol Med.
2017 Nov;49(11):e401. 10.1038/emm.2017.191.
Trib2 regulates the pluripotency of embryonic stem cells and enhances reprogramming efficiency
- Affiliations
-
- 1Department of Physiology, School of Medicine, Pusan National University, Yangsan, Republic of Korea. jhkimst@pusan.ac.kr
- 2UNIST Central Research Facility, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.
- 3Department of Oral Biochemistry and Molecular Biology, School of Dentistry, Pusan National University, Yangsan, Republic of Korea.
- 4Functional Genomics Research Center, KRIBB, Daejeon, Republic of Korea.
- 5Research Institute of Convergence Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Republic of Korea.
- KMID: 2396806
- DOI: http://doi.org/10.1038/emm.2017.191
Abstract
- Embryonic stem (ES) cells are pluripotent cells characterized by self-renewability and differentiation potential. Induced pluripotent stem (iPS) cells are ES cell-equivalent cells derived from somatic cells by the introduction of core reprogramming factors. ES and iPS cells are important sources for understanding basic biology and for generating therapeutic cells for clinical applications. Tribbles homolog 2 (Trib2) functions as a scaffold in signaling pathways. However, the relevance of Trib2 to the pluripotency of ES and iPS cells is unknown. In the present study, we elucidated the importance of Trib2 in maintaining pluripotency in mouse ES cells and in generating iPS cells from somatic cells through the reprogramming process. Trib2 expression decreased as ES cells differentiated, and Trib2 knockdown in ES cells changed their colony morphology while reducing the activity of alkaline phosphatase and the expression of the pluripotency marker genes Oct4, Sox2, Nanog and Klf4. Trib2 directly interacted with Oct4 and elevated Oct4 promoter activity. During the generation of iPS cells, Trib2 knockdown decreased the reprogramming efficiency of mouse embryonic fibroblasts, whereas Trib2 overexpression significantly increased their reprogramming efficiency. In summary, our results suggest that Trib2 is important for maintaining self-renewal in ES cells and for pluripotency induction during the reprogramming process.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Delivering Factors for Reprogramming a Somatic Cell to Pluripotency
- Dental-derived cells for regenerative medicine: stem cells, cell reprogramming, and transdifferentiation
- Inhibition of Class I Histone Deacetylase Enhances Self-Reprogramming of Spermatogonial Stem Cells into Pluripotent Stem Cells
- Mbd3 Promotes Reprogramming of Primary Human Fibroblasts
- Trends and clinical application of induced pluripotent stem cells
