Tissue Eng Regen Med.  2017 Jun;14(3):287-296. 10.1007/s13770-017-0035-4.

Evaluation of Platelet-Rich Plasma and Neutrophil Antimicrobial Extract as Two Autologous Blood-Derived Agents

• Szponder T
• Wessely-Szponder J
• Smolira A

• KMID: 2395085
• DOI: http://doi.org/10.1007/s13770-017-0035-4

Abstract

The platelet-rich plasma (PRP) and antimicrobial peptides neutrophil extract (AMP extract) were prepared from rabbit neutrophils as two autologous blood-derived preparations, which could be applied locally to enhance healing process of tissues. Both preparations were analyzed using the MALDI TOF method for accurate qualitative assay. Growth factors (PDGF and VEGF) and microbicidal protein were reported in PRP. In AMP extract a-defensins, namely; NP-1, -2, -3a, -3b, -4, and -5 and cathelicidins represented among other by 15-kDa antibacterial protein (p15s) were detected. In the second part of our study the influence of antimicrobial extract on macrophages in vitro was tested. Then, degranulation of neutrophils in vitro and generation of reactive intermediates by these cells under the influence of AMP extract were assessed. As estimated, the addition of AMP extract into cultures of macrophages decreased superoxide anion generation after 5 days of incubation. Furthermore, AMP extract inhibited degranulation and respiratory burst in neutrophils, therefore in this regard it suppress proinflammatory effect of two studied populations of leukocytes.

Keyword

Antimicrobial peptides neutrophil extract; Cathelicidin; Platelet-rich plasma

MeSH Terms

Cathelicidins
In Vitro Techniques
Intercellular Signaling Peptides and Proteins
Leukocytes
Macrophages
Methods
Neutrophils*
Peptides
Platelet-Rich Plasma*
Respiratory Burst
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Superoxides
Cathelicidins
Intercellular Signaling Peptides and Proteins
Peptides
Superoxides