J Biomed Transl Res.  2017 Sep;18(3):84-92. 10.12729/jbtr.2017.18.3.084.

Antimicrobial resistance of Stx2e positive Escherichia coli before and after ban on antibiotic growth promoters

• Do KH
• Byun JW
• Lee WK

• KMID: 2394592
• DOI: http://doi.org/10.12729/jbtr.2017.18.3.084

Abstract

The emergence of antimicrobial resistant Escherichia (E.) coli is a major problem in pig farms. To tackle this issue, in July 2011, the Korean government banned the use of antimicrobials for growth promotion of animals in farms. Moreover, E. coli encoding the Stx2e gene cause edema disease which results in high mortality and morbidity in pig farms. Therefore, the aim of this study was to investigate the prevalence of antimicrobial resistance among E. coli encoding the Stx2e gene isolated from weaned piglets with diarrhea before and after the ban on antibiotic growth promoters (AGPs) in Korea from 2007 to 2016. In this period, 479 E. coli isolates were obtained from weaned piglets with diarrhea, and of them, 144 E. coli isolates encoding the Stx2e gene were detected by polymerase chain reaction. The susceptibility of the E. coli isolates to antibiotics were tested using the standard Kirby- Bauer disk diffusion method. The most frequently observed resistances in isolates obtained from weaned piglets in the last 10 years were to tetracycline (92.4%) and chloramphenicol (88.9%). The prevalence of resistance to colistin (3.1% to 16.5%) and tetracycline (86.2% to 97.5%) was also observed to have increased over this period. Additionally, multi-drug resistance was also found to have increased (87.7% to 97.5%) after the ban on AGPs. These findings provide useful data for designing prevention and treatment strategies for postweaning diarrhea and edema disease, and can be used in future studies on antimicrobial resistance in Korea.

Keyword

Antimicrobial resistance; Escherichia coli; Stx2e; Edema disease; Weaned piglets

MeSH Terms

Agriculture
Animals
Anti-Bacterial Agents
Chloramphenicol
Colistin
Diarrhea
Diffusion
Drug Resistance, Multiple
Edema
Escherichia coli*
Escherichia*
Korea
Methods
Mortality
Polymerase Chain Reaction
Prevalence
Tetracycline
Anti-Bacterial Agents
Chloramphenicol
Colistin
Tetracycline