Nat Prod Sci.  2017 Sep;23(3):192-200. 10.20307/nps.2017.23.3.192.

Antioxidant and Antiaging Assays of Hibiscus sabdariffa Extract and Its Compounds

• Widowati W
• Rani AP
• Hamzah RA
• Arumwardana S
• Afifah E
• Kusuma HS
• Rihibiha D
• Nufus H
• Amalia A

• KMID: 2393800
• DOI: http://doi.org/10.20307/nps.2017.23.3.192

Abstract

Skin aging is a complex biological process due to intrinsic and extrinsic factors. Free radical oxidative is one of extrinsic factors that induce activation of collagenase, elastase and hyaluronidase. Natural product from plants has been used as antioxidant and antiaging. This study aimed to evaluate antioxidant and antiaging properties of Hibiscus sabdariffa extract (HSE) and its compounds including myricetin, ascorbic acid, and β carotene. The phytochemical of H. sabdariffa was determined using modified Farnsworth method and presence of phenols, flavonoids and tannins were in moderate content, whereas triterpenoids and alkaloids were in low content. Total phenolic content performed using Folin-Ciocalteu method, was 23.85 µg GAE/mg. Quantitative analysis of myricetin, β-carotene, and ascorbic acid of HSE was performed with Ultra-High Performance Liquid Chromatography (UHPLC) that shows 78.23 µg/mg myricetin, 0.034 µg/mg β-carotene, whilst ascorbic acid was not detected. HSE has lower activity on DPPH (ICâ‚…â‚€ = 195.73 µg/mL) compared to β-carotene, the lowest in ABTS assay (IC50 = 74.58 µg/mL) and low activity in FRAP assay (46.24 µM Fe(II)/µg) compared to myricetin, β-carotene. Antiaging was measured through inhibitory activity of collagenase, elastase, and hyaluronidase. HSE had weakest collagenase inhibitory activity (ICâ‚…â‚€= 750.33 µg/mL), elastase inhibitory activity (103.83 µg/mL), hyaluronidase inhibitory activity (ICâ‚…â‚€ = 619.43 µg/mL) compared to myricetin, β-carotene, and ascorbic acid. HSE contain higher myricetin compared to β-carotene. HSE has moderate antioxidants and lowest antiaging activities. Myricetin is the most active both antioxidant and antiaging activities.

Keyword

Hibiscus sabdariffa; Myricetin; β-carotene; Ascorbic acid; Antioxidant; Antiaging

MeSH Terms

Alkaloids
Antioxidants
Ascorbic Acid
Biological Processes
Carotenoids
Chromatography, Liquid
Collagenases
Flavonoids
Hibiscus*
Hyaluronoglucosaminidase
Methods
Pancreatic Elastase
Phenol
Phenols
Skin Aging
Tannins
Alkaloids
Antioxidants
Ascorbic Acid
Carotenoids
Collagenases
Flavonoids
Hyaluronoglucosaminidase
Pancreatic Elastase
Phenol
Phenols
Tannins

Figure

• 

  Fig. 1 Chemical structure of compounds in the H. sabdariffa. (1) Myricetin (C15H10O8), (2) Ascorbic acid (C6H8O6), (3) β-carotene (C40H56).

• 

  Fig. 2 Chromatogram of UHPLC Analysis. (1) HSE and myricetin, (2) HSE and β-carotene, (3) HSE and ascorbic acid.

• 

  Fig. 3 Effect of HSE (µg/mL), myricetin, β-carotene, ascorbic acid (µM) on collagenase, elastase, and hyaluronidase inhibitory activity. (1) Collagenase inhibitory activity, (2) Elastase inhibitory activity, (3) Hyaluronidase inhibitory activity.

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