Ann Surg Treat Res.  2017 Jul;93(1):1-10. 10.4174/astr.2017.93.1.1.

Omega-3 fatty acids inhibit oxidative stress in a rat model of liver regeneration

• Firat O
• Makay O
• Yeniay L
• Gokce G
• Yenisey C
• Coker A

• KMID: 2392277
• DOI: http://doi.org/10.4174/astr.2017.93.1.1

Abstract

PURPOSE
Lipid peroxidation and consequent reactive oxygen species in the setting of oxidative stress have crucial roles in liver regeneration, which may adversely affect the regeneration itself and lead to liver failure. The aim of the current study is to investigate whether omega-3 fatty acid supplementation inhibits oxidative stress in an experimental model of liver regeneration.
METHODS
Forty rats were allocated to four groups. Rats in group A received a sham operation. Rats in group B were subjected to right portal vein ligation (RPVL) and saline infusion. Rats in groups C and D were subjected to RPVL and total parenteral nutrition (TPN) with an all-in-one admixture containing a soybean oil based lipid emulsion. Rats in group D were additionally supplemented with omega-3 fatty acid infusion. Oxidative stresses in the blood and liver were measured by glutathione, superoxide dismutase, catalase, glutathione peroxidase, malondialdehyde, and nitric oxide.
RESULTS
Omega-3 supplementation to the TPN solution significantly corrected alterations in the blood and tissue concentrations of oxidants and anti-oxidants during regeneration (P < 0.05).
CONCLUSION
Omega-3 fatty acid supplementation to the TPN solution revealed promising results in removal of oxidative stress that emerges during liver regeneration.

Keyword

Omega-3 fatty acids; Oxidative stress; Liver regeneration

Figure

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  Fig. 1 The alterations in the blood concentrations of the oxidants and the components of the antioxidant defense system. MDA, malondialdehyde; NO, nitric oxide; GSH, glutathione; SOD, superoxide dismutase; GPx, glutathione peroxidase. MDA: P = 0.0051 for group C vs. A, P = 0.0075 for group C vs. D, P = 0.0057 for group D vs. B, P = 0.25 for group C vs. B, and P = 0.39 for group D vs. A; NO: P = 0.065 for group C vs. A, P = 0.029 for group C vs. D, and P = 0.066 for group D vs. B, P = 0.27 for group C vs. B, and P = 0.73 for group D vs. A. GSH: P = 0.00031 for group A vs. B, P = 0.00042 for group A vs. C, P = 0.00019 for group B vs. D, P = 0.0084 for group D vs. C, P = 0.63 for group A vs. D, and P = 0.28 for group B vs. C; Catalase: P = 0.00041 for group A vs. B, P = 0.00082 for group A vs. C, P = 0.00059 for group B vs. D, P = 0.0093 for group D vs. C, P = 0.52 for group A vs. D, and P = 0.47 for group B vs. C; SOD: P = 0.00021 for group A vs. B, P = 0.00032 for group A vs. C, P = 0.00039 for group B vs. D, P = 0.0054 for group D vs. C, P = 0.16 for group A vs. D, and P = 0.25 for group B vs. C; GPx: P = 0.00071 for group A vs. B, P = 0.00034 for group A vs. C, P = 0.00062 for group B vs. D, P = 0.00022 for group D vs. C, P = 0.17 for group A vs. D, and P = 0.27 for group B vs. C.

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  Fig. 2 The alterations in the tissue concentrations of the oxidants and the components of the antioxidant defense system for the right and left lobes separately. The X plots represent the right lobes where the Y plots represent the left lobes. MDA, malondialdehyde; NO, nitric oxide; GSH, glutathione; SOD, superoxide dismutase; GPx, glutathione peroxidase. MDA: P = 0.00011 for group A vs. B, P = 0.00032 for group A vs. C, P = 0.00047 for group B vs. D, P = 0.0021 for group C vs. D, P = 0.17 for group A vs. D, and P = 0.66 for B vs. C; NO: P = 0.00069 for group A vs. B, P = 0.00058 for group A vs. C, P = 0.00036 for group B vs. D, P = 0.0021 for group C vs. D, P = 0.43 for group A vs. D, and P = 0.26 for group B vs. C; GSH: P = 0.00021 for group A vs. B, P = 0.00077 for group A vs. C, P = 0.00047 for group B vs. D, P = 0.00035 for group C vs. D, P = 0.12 for group A vs. D, and P = 0.24 for group B vs. C. Catalase: P = 0.0003 for group A vs. B, P = 0.00071 for group A vs. C, P = 0.00033 for group B vs. D, P = 0.00025 for group C vs. D; P = 0.36 for group A vs. D, and P = 0.53 for group B vs. C; SOD: P = 0.00014 for group A vs. B, P = 0.0061 for group A vs. C, P = 0.0023 for group B vs. D, P = 0.027 for group C vs. D, P = 0.21 for group A vs. D, and P = 0.36 for group B vs. C; GPx: P = 0.00029 for group A vs. B, P = 0.00035 for group A vs. C, P = 0.037 for group B vs. D, P = 0.044 for group C vs D, P = 0.65 for group A vs. D, and P = 0.26 for group B vs. C.

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