J Gastric Cancer.  2016 Dec;16(4):221-229. 10.5230/jgc.2016.16.4.221.

Dysregulation of MicroRNA-196b-5p and MicroRNA-375 in Gastric Cancer

• Lee SW
• Park KC
• Kim JG
• Moon SJ
• Kang SB
• Lee DS
• Sul HJ
• Ji JS
• Jeong HY

• KMID: 2389847
• DOI: http://doi.org/10.5230/jgc.2016.16.4.221

Abstract

PURPOSE
Dysregulated microRNAs (miRNAs) can contribute to cancer development by leading to abnormal proliferation of cells, apoptosis, and differentiation. Although several miRNAs that are related to gastric cancer have been identified, the reported results have been inconsistent. The aim of this study was to determine miRNA expression profiles and validate miRNAs up- and down-regulated in gastric cancer.
MATERIALS AND METHODS
We evaluated 34 primary gastric cancer tissues and paired adjacent nontumorous gastric tissues. Total RNA was extracted, and low-molecular-weight RNAs (<200 nucleotides) were isolated for further analysis. Two pairs of tissues were processed for GeneChip microarray analysis, and the identified up- and down-regulated miRNAs were validated by real-time quantitative polymerase chain reaction (qPCR).
RESULTS
In the set of differentially expressed miRNAs, 5 were overexpressed by more than 2 fold, and 5 were reduced by 2 fold or less in gastric cancer tissues compared with normal gastric tissues. Four of these miRNAs (miR-196b-5p, miR-375, miR-483-5p, and miR-486-5p) were then validated by qPCR, and the relative expression levels of 2 miRNAs (miR-196b-5p and miR-375) were significantly different between cancer and normal tissues.
CONCLUSIONS
Our results revealed that the expression of miR-196b-5p and miR-375 significantly correlates with gastric cancer. These miRNAs could therefore serve as diagnostic biomarkers of gastric cancer.

Keyword

MicroRNAs; Stomach; Neoplasms; Microarray analysis; Polymerase chain reaction

MeSH Terms

Apoptosis
Biomarkers
Microarray Analysis
MicroRNAs
Polymerase Chain Reaction
RNA
Stomach
Stomach Neoplasms*
Biomarkers
MicroRNAs
RNA

Figure

• 

  Fig. 1 Hierarchical clustering analysis of miR expression in gastric cancer and paired normal gastric tissues (GeneChip microarray analysis). Colors indicate relative signal intensities; red and blue indicate increased and repressed expression, respectively. N group = normal group; T group = tumor group; hsa = human micro RNA; miR = microRNA.

• 

  Fig. 2 Gene ontology analysis of the genes related to 10 microRNAs identified in the microarray analysis (P<0.05). The DAVID bioinformatic resource tool was used for the analysis.

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  Fig. 3 Summary of real-time quantitative polymerase chain reaction analysis. The increased (miR-196b-5p and miR-375) and the repressed (miR-483-5p and miR-486-5p) miRNAs are presented. The miR-483-5p and miR-375 results were contrary to the microarray results. Four selected miRNAs are listed on the X-axis, and relative expression levels are indicated on the Y-axis. Two miRNAs (miR-196b-5p and miR-375) demonstrated significantly different expression levels between gastric cancer tissues and adjacent non-tumorous tissues (P<0.05). miR = microRNA.

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