Nat Prod Sci.  2017 Jun;23(2):84-91. 10.20307/nps.2017.23.2.84.

Quantitative Analysis of Eleven Bioactive Constituents of a Traditional Herbal Medicine, Yeonggyechulgam-tang using, Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

• Seo CS
• Shin HK

• KMID: 2387056
• DOI: http://doi.org/10.20307/nps.2017.23.2.84

Abstract

This study proposes a sensitive and selective liquid chromatography-electrospray ionization tandem mass spectrometry method of efficiently assessing the quality of a traditional herbal medicine called Yeonggyechulgam-tang (YGCGT). The following compounds 1 - 11, namely, liquiritin apioside (1), liquiritin (2), liquiritigene (3), coumarin (4), cinnamic acid (5), cinnamaldehyde (6), glycyrrhizin (7), atractylenolide III (8), atractylenolide II (9), atractylenolide I (10), and pachymic acid (11) were separated on a UPLC BEH C₁₈ column (2.1 × 100 mm, 1.7 µm) at a column temperature of 45℃ eluted with a gradient condition of 0.1% (v/v) formic acid in distilled water and acetonitrile. The correlation coefficient of the calibration curve of the eleven constituents was ≥ 0.9936. The limits of detection and quantification of the compounds 1 - 11 were 0.06 - 4.73 ng/mL and 0.17-14.20 ng/mL, respectively. Using this analytical method, the compound 11 in lyophilized YGCGT decoction extract was not detected, while the compounds 1 - 10 were detected 0.13-166.43 mg/g.

Keyword

Quantitative analysis; Yeonggyechulgam-tang; LC-MS/MS

MeSH Terms

Calibration
Glycyrrhizic Acid
Herbal Medicine*
Limit of Detection
Methods
Tandem Mass Spectrometry*
Water
Glycyrrhizic Acid
Water

Figure

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  Fig. 1. Chemical structures of the compounds 1 – 11 in Yeonggyechulgam-tang.

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  Fig. 2. Mass spectra of the precursor ion (Q1, A) and product ion (Q3, B) for LC-MS/MS MRM mode of the compounds 1 – 11. Liquiritin apioside (1), liquiritin (2), liquiritigene (3), coumarin (4), cinnamic acid (5), cinnamaldehyde (6), glycyrrhizin (7), atractylenolide III (8), atractylenolide II (9), atractylenolide I (10), and pachymic acid (11).

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  Fig. 3. Total ion chromatograms of the reference standard (A) and lyophilized Yeonggyechulgam-tang extract (B) by LC-MS/MS MRM mode. Liquiritin apioside (1), liquiritin (2), liquiritigene (3), coumarin (4), cinnamic acid (5), cinnamaldehyde (6), glycyrrhizin (7), atractylenolide III (8), atractylenolide II (9), atractylenolide I (10), and pachymic acid (11, not detected).

Reference

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