Maxillofac Plast Reconstr Surg.
2015 Jun;37(6):16. 10.1186/s40902-015-0015-3.
Combined effect of bisphosphonate and recombinant human bone morphogenetic protein 2 on bone healing of rat calvarial defects
- Affiliations
-
- 1Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, 626-787 Yangsan, Mulgeum-eup, Korea. ssh8080@pusan.ac.kr
- 2Department of Oral Anatomy and Cell Biology, School of Dentistry, Pusan National University, Yangsan, Korea.
- 3Biomedical Research Institute of Pusan National University, Yangsan, Korea.
- 4Department of Oral and Maxillofacial Surgery, Good Gang-An Hospital, Busan, Korea.
- KMID: 2381926
- DOI: http://doi.org/10.1186/s40902-015-0015-3
Abstract
- PURPOSE
This study aimed to investigate new bone formation using recombinant human bone morphogenetic protein 2 (rhBMP-2) and locally applied bisphosphonate in rat calvarial defects.
METHODS
Thirty-six rats were studied. Two circular 5 mm diameter bony defect were formed in the calvaria using a trephine bur. The bony defect were grafted with Bio-Oss(R) only (group 1, n = 9), Bio-Oss(R) wetted with rhBMP-2 (group 2, n = 9), Bio-Oss(R) wetted with rhBMP-2 and 1 mM alendronate (group 3, n = 9) and Bio-Oss(R) wetted with rhBMP-2 and 10 mM alendronate (group 4, n = 9). In each group, three animals were euthanized at 2, 4 and 8 weeks after surgery, respectively. The specimens were then analyzed by histology, histomorphometry and immunohistochemistry analysis.
RESULTS
There were significant decrease of bone formation area (p < 0.05) between group 4 and group 2, 3. Group 3 showed increase of new bone formation compared to group 2. In immunohistochemistry, collagen type I and osteoprotegerin (OPG) didn't show any difference. However, receptor activator of nuclear factor kappaB ligand (RANKL) decreased with time dependent except group 4.
CONCLUSION
Low concentration bisphosphonate and rhBMP-2 have synergic effect on bone regeneration and this is result from the decreased activity of RANKL of osteoblast.
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