Korean J Phys Anthropol.  1996 Dec;9(2):189-195. 10.11637/kjpa.1996.9.2.189.

Effect of Allopurinol on Methylmercuric Chloride in Cultured O1igodendrocytes

• Park ST
• Mun YJ
• Oh JM
• Kim JJ
• Choi MK
• Shim JH
• Lim KT
• Chung YT

• KMID: 2376319
• DOI: http://doi.org/10.11637/kjpa.1996.9.2.189

Abstract

In order to examine the neurotoxic mechanism of oxygen radicals on cultured bovine oligodendrocytes, cytoxic effect of oxygen radicals was examined when cultures were treated with various concentrations of xanthine oxidase (XO) and hypoxanthine (HX) in culture medium. In addition, the neuroprotective effect of iron-chelators against the neurotoxicity induced by oxygen radicals was evaluated by MTT assay. Cell viability was remarkably decreased in a time-dependent manner after exposure of cultured bovine oligodendrocytes to 20mU/ml XO and 0.1mM HX for 4 hours. In the neuroprotective effect of iron-chelators on oxidant-induced neurotoxicity, tetrakis (2-pyridylmethyl)ethylenediamine (TPEN) blocked the neurotoxicity induced by oxygen radicals, while DFX was not effective in blocking oxidant-induced neurotoxicity in these cultures. These results suggest that oxygen radicals are toxic in cultured bovine oligodendrocytes, and also selective iron-chelators such as TPEN are effective in blocking the neurotoxicity induced by oxygen radicals.

Keyword

Iron-chelator; Neurotoxicity; Oxygen radical; ultured bovine oligodendrocyte

MeSH Terms

Allopurinol*
Cell Survival
Hypoxanthine
Neuroprotective Agents
Oligodendroglia
Reactive Oxygen Species
Xanthine Oxidase
Allopurinol
Hypoxanthine
Neuroprotective Agents
Reactive Oxygen Species
Xanthine Oxidase