Ann Lab Med.  2016 Sep;36(5):501-503. 10.3343/alm.2016.36.5.501.

MLL-SEPT5 Fusion Transcript in Two de novo Acute Myeloid Leukemia Patients With t(11;22)(q23;q11)

• Wang N
• Wu X
• Sheng G
• Ma L
• Wen L
• Yao H
• Chen S

• KMID: 2373585
• DOI: http://doi.org/10.3343/alm.2016.36.5.501

Abstract

No abstract available.

MeSH Terms

Base Sequence
Cell Cycle Proteins/*genetics
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 22
Female
Gene Rearrangement
Histone-Lysine N-Methyltransferase/*genetics
Humans
Immunophenotyping
In Situ Hybridization, Fluorescence
Karyotype
Leukemia, Myeloid, Acute/*diagnosis/metabolism
Male
Myeloid-Lymphoid Leukemia Protein/*genetics
Oncogene Proteins, Fusion/genetics
Reverse Transcriptase Polymerase Chain Reaction
Septins/*genetics
Sequence Analysis, DNA
Translocation, Genetic
Young Adult
Cell Cycle Proteins
Histone-Lysine N-Methyltransferase
Oncogene Proteins, Fusion
Myeloid-Lymphoid Leukemia Protein
Septins

Figure

• 

  Fig. 1 FISH analysis using 11q23 break-apart probe revealed MLL rearrangement. Separation of a green and a red signal was observed.Abbreviation: MLL, mixed lineage leukemia.

• 

  Fig. 2 Identification of the MLL-SEPT5 fusion transcripts by reverse transcription-PCR. Lane M, 1kb DNA ladder; Lane E, empty control; Lane N, PCR control; Lane P1, patient 1; Lane P2, patient 2.Abbreviation: MLL, mixed lineage leukemia.

• 

  Fig. 3 Sequences of junction of the MLL-SEPT5 chimeric transcript. (A) Patient 1, (B) patient 2.Abbreviation: MLL, mixed lineage leukemia.

Reference

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