Ann Lab Med.  2016 May;36(3):263-265. 10.3343/alm.2016.36.3.263.

Minor BCR-ABL1-Positive Acute Myeloid Leukemia Associated With the NPM1 Mutation and FLT3 Internal Tandem Duplication

• Kim MJ
• Ahn S
• Jeong SH
• Jang JH
• Han JH
• Choi JR
• Cho SR

• KMID: 2373538
• DOI: http://doi.org/10.3343/alm.2016.36.3.263

Abstract

No abstract available.

MeSH Terms

Aged
Base Sequence
Bone Marrow/metabolism/pathology
DNA Mutational Analysis
Female
Fusion Proteins, bcr-abl/*genetics
Gene Duplication
Humans
In Situ Hybridization, Fluorescence
Leukemia, Myeloid, Acute/diagnosis/*genetics
Multiplex Polymerase Chain Reaction
Mutation
Nuclear Proteins/*genetics
Philadelphia Chromosome
fms-Like Tyrosine Kinase 3/*genetics
Fusion Proteins, bcr-abl
Nuclear Proteins
fms-Like Tyrosine Kinase 3

Figure

• 

  Fig. 1 Bone marrow findings of the patient. (A) Aspirate smear showing increased blasts (Wright-Giemsa, ×1,000). The blasts with cup-like nuclei and fish mouth nuclei (inlet) are observed. (B) Biopsy section showing a diffuse infiltration by the blasts (Hematoxylin and eosin, ×200).

• 

  Fig. 2 Sequencing analyses show the presence of type A mutation (c.863_864insTCTG) in the nucleophosmin gene (NPM1) (A) and an FLT3 internal tandem duplication (ITD) with a 45 bp in-frame duplication (c.1788_1832dup) (B). Arrows indicate the insertion sites.

Reference

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