J Gastric Cancer.  2016 Mar;16(1):21-27. 10.5230/jgc.2016.16.1.21.

Prognostic and Clinicopathological Significance of Transducer-Like Enhancer of Split 1 Expression in Gastric Cancer

• Lee JH
• Son MW
• Kim KJ
• Oh MH
• Cho H
• Lee HJ
• Jang SH
• Lee MS

• KMID: 2372549
• DOI: http://doi.org/10.5230/jgc.2016.16.1.21

Abstract

PURPOSE
Transducer-like enhancer of split 1 (TLE1) is a member of the Groucho/TLE family of transcriptional co-repressors that regulate the transcriptional activity of numerous genes. TLE1 is involved in the tumorigenesis of various tumors. We investigated the prognostic significance of TLE1 expression and its association with clinicopathological parameters in gastric cancer (GC) patients.
MATERIALS AND METHODS
Immunohistochemical analysis of six tissue microarrays was performed to examine TLE1 expression using 291 surgically resected GC specimens from the Soonchunhyang University Cheonan Hospital between July 2006 and December 2009.
RESULTS
In the non-neoplastic gastric mucosa, TLE1 expression was negative. In GC, 121 patients (41.6%) were positive for TLE1. The expression of TLE1 was significantly associated with male gender (P=0.021), less frequent lymphatic (P=0.017) or perineural invasion (P=0.029), intestinal type according to the Lauren classification (P=0.024), good histologic grade (P<0.001), early pathologic T-stage (P=0.012), and early American Joint Committee on Cancer stage (P=0.022). In the Kaplan-Meier analysis, the TLE1 expression was significantly associated with longer disease-free (P=0.022) and overall (P=0.001) survival rates.
CONCLUSIONS
We suggested that TLE1 expression is a good prognostic indicator in GCs.

Keyword

TLE1; Stomach neoplasms; Survival; Tissue array analysis

MeSH Terms

Carcinogenesis
Chungcheongnam-do
Classification
Co-Repressor Proteins
Gastric Mucosa
Humans
Joints
Kaplan-Meier Estimate
Male
Stomach Neoplasms*
Survival Rate
Tissue Array Analysis
Co-Repressor Proteins

Figure

• 

  Fig. 1 Immunohistochemical (IHC) staining of transducer-like enhancer of split 1 (TLE1) in normal gastric mucosa and gastric cancer (GC). (A) Representative images of TLE1 IHC of normal gastric mucosa (×200) exhibiting a score of 0. (B) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 1. (C) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 2. (D) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 3.

• 

  Fig. 2 Kaplan-Meier survival analysis with log-rank test. Transducer-like enhancer of split 1 expression was significantly associated with good overall survival (A) and good disease-free survival (B).

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