Investig Clin Urol.  2017 Mar;58(2):140-145. 10.4111/icu.2017.58.2.140.

Evaluation of zinc finger E-box binding homeobox 1 and transforming growth factor-beta2 expression in bladder cancer tissue in comparison with healthy adjacent tissue

• Mahdavinezhad A
• Yadegarazari R
• Mousavi-Bahar SH
• Poorolajal J
• Jafari M
• Amirzargar MA
• Effatpanah H
• Saidijam M

• KMID: 2368480
• DOI: http://doi.org/10.4111/icu.2017.58.2.140

Abstract

PURPOSE
The fifth most common cancer is allocated to bladder cancer (BC) worldwide. Understanding the molecular mechanisms of BC invasion and metastasis to identify target therapeutic strategies will improve disease survival. So the aim of this study was to measure expression rate of zinc finger E-box binding homeobox 1 (ZEB1) and transforming growth factor-beta2 (TGF-β2) mRNA in tissue samples of patients with BC and its healthy adjacent tissue samples and their association with muscle invasion, size and grade of the tumor.
MATERIALS AND METHODS
Tissue samples were collected from 35 newly diagnosed untreated patients with BC from 2013 to 2014. Total RNA was extracted from about 50-mg tissue samples using TRIzol reagent. TAKARA SYBR Premix EX Tag II was applied to determine the rate of mRNA expression by real-time polymerase chain reaction (PCR). To obtain final validation, PCR product of ZEB1 and TGF-β2 were sequenced. STATA 11 software was used to analyze the data.
RESULTS
The expression level of ZEB1 in tumor samples was significantly more than of in healthy adjacent tissue samples. Up-regulation of TGF-β2 showed a strong association with muscle invasion (p=0.017). There was also demonstrated a relationship between over expression of ZEB1 with the tumor size (p=0.050).
CONCLUSIONS
It looks ZEB1 and TGF-β2 had a role in BC patients. In this study ZEB1 expression was higher in BC tissues than that of in healthy control tissues. There was demonstrated a markedly association between overexpression of TGF-β2 and muscle invasion. Therefore, they are supposed to be candidate as potential biomarkers for early detection and progression of BC.

Keyword

Neoplasm grading; Transforming growth factor beta2; Urinary bladder neoplasms; ZEB1 protein

MeSH Terms

Biomarkers
Genes, Homeobox*
Humans
Neoplasm Grading
Neoplasm Metastasis
Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
RNA
RNA, Messenger
Transforming Growth Factor beta2
Up-Regulation
Urinary Bladder Neoplasms*
Urinary Bladder*
Zinc Fingers*
Zinc*
Biomarkers
RNA
RNA, Messenger
Transforming Growth Factor beta2
Zinc

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