J Korean Ophthalmol Soc.  1997 Jul;38(7):1162-1171.

Immunohistochemical Study of Transforming Growth Factor-beta2(TGF-beta2) and Vascular Endothelial Growth Factor(VEGF) after Laser Photocoagulation in the Ocular Tissues of White Rats

• Kim SH
• Kim SD

Abstract

To explain a part of the machanism of regression of new vessels after laser photocoagulation in retinal vascular occlusive diseases causing neovascularization, particularly for diabetic retinopathy, retinal vein occlusion, retinopathy of prematurity, and age-related macular degene- ration, we performed this study. Photocoagulation was done with argon green laser on the right superofrontal retina of the 15 eyes of white rats. On the first, third, and sixth day after laser photocoagulation, both eyes of 5 rats were enucleated and stained with polyclonal anti-transforming growth factor-beta2(Anti-TGF-beta 2) and anti-vascular endothelial growth factor(Anti-VEGF) antibody by immuno peroxidase technique. TGF-beta2 and VEGF were not expressed in retina of normal control eyes. After laser photocoagulation, degree of expression of TGF-beta2 and VEGF increased in 24 hours, only in and adjacent to the photocoagulated area. Thereafter, degree of expression of both factor decreased, especially that of VEGF decreased much more than that of TGF-beta2. To elucidate the exact mechanism, more qualitative and quantitative analysis will be necessary.

Keyword

Laser photocoagulation; Neovascularization; Transforming growth factor-beta2(TGF-beta2); Vascular endothelial growth factor(VEGF)

MeSH Terms

Animals
Argon
Diabetic Retinopathy
Light Coagulation*
Peroxidase
Rats*
Retina
Retinal Vein Occlusion
Retinaldehyde
Retinopathy of Prematurity
Transforming Growth Factor beta2
Vascular Endothelial Growth Factor A
Argon
Peroxidase
Retinaldehyde
Transforming Growth Factor beta2
Vascular Endothelial Growth Factor A