Ann Dermatol.  2014 Jun;26(3):289-295.

Scalded Skin of Rat Treated by Using Fibrin Glue Combined with Allogeneic Bone Marrow Mesenchymal Stem Cells

• Yang Y
• Zhang W
• Li Y
• Fang G
• Zhang K

Abstract

BACKGROUND
It is difficult to achieve satisfactory results with the traditional treatment of large-area skin defects and deep burns.
OBJECTIVE
To test the treatment effect of an active dressing film made of a mixture of fibrin glue and bone marrow mesenchymal stem cells (BMSCs) for repairing burn wounds on the skin of rats.
METHODS
Two scald wounds were made on the back of each rat. A total of 30 scald wounds were randomly divided into 3 groups, with 10 wounds in each group. In the experimental treatment group, the scald wounds were covered with the fibrin glue and BMSC mixture. The wounds of the experimental control group were covered with fibrin glue only. No intervention was administered to the blank control group. Thirty days after treatment, pathological sections were cut from the scalded local tissues of all rats from the 3 groups and observed with a microscope.
RESULTS
The speed of scald wound healing in the experimental treatment group was faster than the other 2 groups. In the experimental treatment group, histopathological analysis revealed that the sebaceous glands showed obviously proliferous at the edge of the new tissue and gradually extended to the deep dermal layer of the new tissue.
CONCLUSION
BMSCs may have an active role in promoting skin tissue repair and generating skin appendages. Allogeneic BMSCs mixed with fibrin glue can contribute to the quick formation of a film-like gel over the scald wounds, which might be of significance for emergency treatment and skin-grafting operations.

Keyword

Artificial skin; Burns; Fibrin tissue adhesive; Mesenchyma stromal cells; Tissue engineering

MeSH Terms

Animals
Bandages
Bone Marrow*
Burns
Emergency Treatment
Fibrin Tissue Adhesive*
Mesenchymal Stromal Cells*
Rats*
Sebaceous Glands
Skin*
Skin, Artificial
Tissue Engineering
Wound Healing
Wounds and Injuries
Fibrin Tissue Adhesive

Figure

• 

  Fig. 1 (A) Cell cycle analysis: 86.99% of the cells were in the G0/G1 phase, 6.76% were in the synthesis phase (S phase), and 6.25% were in the G2/M phase. (B) In vitro differentiation assay, von Kossa staining, and toluidine blue staining of rat bone marrow mesenchymal stem cells (BMSCs). (a) The negative controls (uninduced BMSCs) did not stain positive for von Kossa dye (×100). (b) After 21 days of osteogenic induction, the cells showed positive von Kossa staining (×100). (c) The negative control BMSCs were negative for toluidine blue staining (×100). (d) After the BMSCs were chondrogenically induced for 28 days, they showed positive toluidine blue staining (×40).

• 

  Fig. 2 Skin changes of the scald skin area at different times. (A) Ten minutes after scalding, (B) 1 day after scalding, and (C) 4 days after scalding.

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  Fig. 3 Changes in wound diameter at different times after transplantation. *Compared with the experimental treatment group, p<0.01.

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  Fig. 4 Hematoxylin and eosin staining of the newly formed tissue in the 3 groups of rats 30 days after transplantation. The epidermal layer of each figure was marked with red arrow. (A) Normal skin: The epidermal layer (red arrows) was significantly thinner than in the other groups. There are abundant collagen and elastic fibers in the dermal layer, and they are disorderly arranged. Many skin appendages, such as sebaceous glands, hair follicles, and sweat glands, are also present (×100). (B) Blank control group. There are no elastic fiber and skin appendages in the dermal layer (×100). (C) Experimental control group (×100). (D) Experimental treatment group. Sebaceous glands show obviously proliferous at the edge of the new tissue, and gradually extend to the deep dermal layer. The epidermal layer recessed and formed a hair follicle-like structure (black arrows) (×100).

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