J Korean Orthop Res Soc.
2015 Dec;18(2):43-50. 10.0000/jkors.2015.18.2.43.
In Vivo Cartilage Formation Using Human Bone Marrow-Derived Mesenchymal Stem Cells Mixed with Fibrin Glue
- Affiliations
-
- 1Brain Korea 21 Project Plus for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
- 2Department of Orthopedic Surgery, Yonsei University College of Medicine, Seoul, Korea. orthohwan@gmail.com
- KMID: 2162810
- DOI: http://doi.org/10.0000/jkors.2015.18.2.43
Abstract
- PURPOSE
To investigate the feasibility of in vivo cartilage formation by direct injection the chondrogenic undifferentiated human bone marrow-derived mesenchymal stem cells (hBMSCs) mixed with fibrin glue including TGF-beta3.
MATERIALS AND METHODS
Chondrogenic differentiation induced hBMSCs for 14 days (control group-2) and undifferentiated hBMSCs combined with TGF-beta3 mixed (experimental group-3) with fibrin glue and fibrin glue only (control group-1) were injected subcutanteously into the back of nude mouse. For evaluation of the cartilage-like tissue formed after 8 weeks after injection, real time PCR, histological analysis and immunohistochemical analysis were used.
RESULTS
Control group-1 did not form any visible mass. Control group-2 as well as experimental group-3 could form new cartilage-like tissue which were demonstrated expression of type II collagen by real-time PCR, histology analysis such as H&E staining, MT staining and type II collagen specific immunohistologic analysis. As results, expression of type II collagen was shown in the both groups.
CONCLUSION
Our study confirmed that cartilage-like tissues could be formed in subcutaneous layer of nude mouse by direct injection mixed with fibrin glue including TGF-beta3 without chondrogenic-induction of hBMSCs, suggesting that these model could be suitable for preliminary studies or optimizing experiments to evaluate reconstruction of cartilage.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Scalded Skin of Rat Treated by Using Fibrin Glue Combined with Allogeneic Bone Marrow Mesenchymal Stem Cells
- The effects of Bio-Oss(R) as a scaffolds during sinus bone graft using mesenchymal stem cells in rabbit
- Exploring upregulated genes during osteogenic differentiation of hMSCs
- Bone Marrow-Derived Mesenchymal Stem Cells for Regenerative Medicine
- The effects of undifferentiated mesenchymal stem cells on sinus bone grafting in rabbit
