Ann Dermatol.  2016 Dec;28(6):718-724. 10.5021/ad.2016.28.6.718.

Histologic Evidence of New Collagen Formulation Using Platelet Rich Plasma in Skin Rejuvenation: A Prospective Controlled Clinical Study

Affiliations
  • 1Department of Dermatology, Acıbadem Fulya Hospital, Istanbul, Turkey.
  • 2Department of Dermatology, Eskisehir Military Hospital, Eskisehir, Turkey. hamzayildiz@gmail.com
  • 3Department of Pathology, Eskisehir Military Hospital, Eskisehir, Turkey.
  • 4Department of Pathology, Anadolu Medical Center, Kocaeli, Turkey.
  • 5Department of Dermatology, Yunus Emre Government Hospital, Eskisehir, Turkey.
  • 6Department of Dermatology, GATA Haydarpasa Teaching Hospital, Istanbul, Turkey.

Abstract

BACKGROUND
Platelet-rich plasma (PRP) is an autologous concentration of human platelets contained in a small volume of plasma and has recently been shown to accelerate rejuvenate aging skin by various growth factors and cell adhesion molecules.
OBJECTIVE
This study was conducted to evaluate the efficacy and safety of intradermal injection of PRP in the human facial rejuvenation.
METHODS
This study was a prospective, single-center, single-dose, open-label, non-randomized controlled clinical study. PRP injected to the upper site of this right infra-auricular area and all face. Saline was injected to the left infra-auricular area. Histopathological examinations were performed before PRP treatment, 28 days after the PRP, and saline (control) treatments.
RESULTS
Twenty women ranging in age from 40 to 49 years (mean age, 43.65±2.43 years) were enrolled in the study. The mean optical densities (MODs) of collagen in the pre-treatment, control, and PRP-treated area were measured. They were 539±93.2, 787±134.15, 1,019±178, respectively. In the MOD of PRP, 89.05 percent improvement was found when MOD of PRP was compared with MOD of pre-treatment. The mean MOD of collagen fibers was clearly highest on the PRP side (p<0.001). The PRP-to-saline improvement ratio (89.05% to 46.01%) was 1.93:1. No serious side effects were detected.
CONCLUSION
PRP increases dermal collagen levels not only by growth factors, but also by skin needling (the mesotherapy technique 'point by point'). PRP application could be considered as an effective (even a single application) and safety procedure for facial skin rejuvenation.

Keyword

Collagen; Mesotherapy; Platelet-rich plasma; Skin needling; Skin rejuvenation

MeSH Terms

Aging
Blood Platelets*
Cell Adhesion Molecules
Collagen*
Female
Humans
Injections, Intradermal
Intercellular Signaling Peptides and Proteins
Mesotherapy
Plasma
Platelet-Rich Plasma*
Prospective Studies*
Rejuvenation*
Skin*
Cell Adhesion Molecules
Collagen
Intercellular Signaling Peptides and Proteins

Figure

  • Fig. 1 The first biopsy was obtained from the right infra-auricular area before treatment (A). Saline was injected to the left infra-auricular area (B). Platelet-rich plasma (PRP) (C) was injected to the upper site of this right infra-auricular area (A) and face (F). On day 28 after PRP and saline injections, punch biopsy was performed on PRP (C) injected to the upper site of this right infra-auricular area (D) and control (saline injected area) site (E).

  • Fig. 2 (A) Photography of papillary and reticular dermis of the face skin, stained by Masson's trichrome, collagen fibers stained blue, ×100. (B1) In the image analysis system, the continuous tone of blue color pixels, which show the presence of collagen, was chosen in the target area. (B2) All of the pixels were converted into numeric values from zero to 15 in the blue spectrum. The sum of the values in the target area represents the integrated optical density, which displayed the collagen area (B1, B2: Masson's trichrome, ×100).

  • Fig. 3 Periorbital wrinkles before platelet-rich plasma (PRP) treatment and clinical improvement of periorbital wrinkles after PRP treatment.

  • Fig. 4 Photography of collagen fibers, stained by Masson's trichrome, collagen fibers stained blue, ×40. (A) Pre-treatment site. (B) Saline injected site. (C) Platelet-rich plasma injected site.


Cited by  2 articles

Re: Histologic Evidence of New Collagen Formulation Using Platelet Rich Plasma in Skin Rejuvenation: A Prospective Controlled Clinical Study
Levent Tekin
Ann Dermatol. 2018;30(1):110-111.    doi: 10.5021/ad.2018.30.1.110.

Re: Histologic Evidence of New Collagen Formulation Using Platelet Rich Plasma in Skin Rejuvenation: A Prospective Controlled Clinical Study: Authors' Reply
Ozlem Karabudak Abuaf, Hamza Yildiz, Hüseyin Baloglu, Memet Ersan Bilgili, Hasan Aktug Simsek, Bilal Dogan
Ann Dermatol. 2018;30(1):111-111.    doi: 10.5021/ad.2018.30.1.111.


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