J Cancer Prev.  2016 Sep;21(3):194-200. 10.15430/JCP.2016.21.3.194.

Quantitative Analysis of Exosomes From Murine Lung Cancer Cells by Flow Cytometry

• Rim KT
• Kim SJ

• KMID: 2367578
• DOI: http://doi.org/10.15430/JCP.2016.21.3.194

Abstract

In vivo studies regarding biochemical, molecular biological, and histopathological changes in cancer tissues have been widely performed by the administration of carcinogens in rodents. In these established methods, dissection of the animal following sacrifice must be carried out. Exosomes are cell-derived vesicles that are present in all body fluids and these vesicles have specific roles within cells. Thus, much attention is given to the clinical application of exosomes that can possibly be used for prediction and therapy and as biomarkers related to cancer. To develop a new tool for monitoring in vivo genetic alterations, as a result of carcinogenesis, without the need for frequent euthanasia, we performed quantitative measurement of exosomes in Mlg2908 murine lung fibroblasts and LA-4 and KLN 205 murine lung cancer cells using fluorescence-activated cell sorting. We detected an increase in CD63-specific exosomes in LA-4 lung cancer cells. This result is able to be applied to the classification of cancer-specific proteins and miRNA as diagnostic markers.

Keyword

Quantitative; Analysis; Exosomes; Flow cytometry; Lung

MeSH Terms

Animals
Biomarkers
Body Fluids
Carcinogenesis
Carcinogens
Classification
Euthanasia
Exosomes*
Fibroblasts
Flow Cytometry*
Lung Neoplasms*
Lung*
MicroRNAs
Rodentia
Biomarkers
Carcinogens
MicroRNAs