Tuberc Respir Dis.  2016 Oct;79(4):282-288. 10.4046/trd.2016.79.4.282.

Performance of the BacT Alert 3D System Versus Solid Media for Recovery and Drug Susceptibility Testing of Mycobacterium tuberculosis in a Tertiary Hospital in Korea

• Kim SC
• Jeon BY
• Kim JS
• Choi IH
• Kim J
• Woo J
• Kim S
• Lee HW
• Sezim M
• Cho SN

• KMID: 2365320
• DOI: http://doi.org/10.4046/trd.2016.79.4.282

Abstract

BACKGROUND
Tuberculosis (TB) is a major health problem, and accurate and rapid diagnosis of multidrug-resistant (MDR) and extended drug-resistant (XDR) TB is important for appropriate treatment. In this study, performances of solid and liquid culture methods were compared with respect to MDR- and XDR-TB isolate recovery and drug susceptibility testing.
METHODS
Sputum specimens from 304 patients were stained with Ziehl-Neelsen method. Mycobacterium tuberculosis (Mtb) isolates were tested for recovery on Löwenstein-Jensen (LJ) medium and the BacT Alert 3D system. For drug susceptibility testing of Mtb, isolates were evaluated on M-KIT plates and the BacT Alert 3D system.
RESULTS
The recovery rates were 94.9% (206/217) and 98.2% (213/217) for LJ medium and the BacT Alert 3D system, respectively (kappa coefficient, 0.884). The rate of drug resistance was 13.4% for at least one or more drugs, 6.0% for MDR-TB and 2.3% for XDR-TB. M-KIT plate and BacT 3D Alert 3D system were comparable in drug susceptibility testing for isoniazid (97.7%; kappa coefficient, 0.905) and rifampin (98.6%; kappa coefficient, 0.907). Antibiotic resistance was observed using M-KIT plates for 24 of the total 29 Mtb isolates (82.8%).
CONCLUSION
The liquid culture system showed greater reduction in the culture period, as compared with LJ medium; however, drug susceptibility testing using M-KIT plates was advantageous for simultaneous testing against multiple drug targets.

Keyword

Tuberculosis; Drug Susceptibility; Mycobacterium tuberculosis; Tertiary Care Center; Korea

MeSH Terms

Diagnosis
Drug Resistance
Drug Resistance, Microbial
Extensively Drug-Resistant Tuberculosis
Humans
Isoniazid
Korea*
Methods
Mycobacterium tuberculosis*
Mycobacterium*
Rifampin
Sputum
Tertiary Care Centers*
Tuberculosis
Isoniazid
Rifampin

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