J Dent Hyg Sci.  2016 Feb;16(1):70-76. 10.17135/jdhs.2016.16.1.70.

A Biocompatibility Evaluation of Hydroxyapaite·Titania Surface for Dental Implant

• Kang MK
• Bae SS

• KMID: 2360846
• DOI: http://doi.org/10.17135/jdhs.2016.16.1.70

Abstract

The objective of this study was to fabricate hydroxyapatite (HA) containing titania layer by HA blasting and anodization method to obtain advantages of both methods and evaluated biocompatibility. To fabricate the HA containing titania layer on titanium, HA blasting treatment was performed followed by microarc oxidation (MAO) using the electrolyte solution of 0.04 M β-glycerol phosphate disodium salt n-hydrate and 0.4 M calcium acetate n-hydrate on the condition of various applied voltages (100, 150, 200, 250 V) for 3 minutes. The experimental group was divided according to the surface treatment procedure: SM (simple machined polishing treatment), HA, MAO, HA+MAO 100, HA+MAO 150, HA+MAO 200, HA+ MAO 250. The wettability of surface was observed by contact angle measurement. Biocompatibility was evaluated by cell adhesion, and cell differentiation including alkaline phosphatase activity and calcium concentration with MC3T3-E1 cells. The porous titanium oxide containing HA was formed at 150 and 200 V. These surfaces had a more hydrophilic characteristic. Biocompatibility was demonstrated that HA·titania composite layer on titanium showed enhanced cell adhesion, and cell differentiation. Therefore, these results suggested that HA containing titania layer on titanium was improved biological properties that could be applied as material for dental implant system.

Keyword

Anodization; Blasting; Dental implants; Hydroxyapatite; Titania

MeSH Terms

Alkaline Phosphatase
Calcium
Cell Adhesion
Cell Differentiation
Dental Implants*
Durapatite
Methods
Monoamine Oxidase
Titanium
Wettability
Alkaline Phosphatase
Calcium
Dental Implants
Durapatite
Monoamine Oxidase
Titanium