Nat Prod Sci.  2016 Sep;22(3):175-179. 10.20307/nps.2016.22.3.175.

Beneficial Effect of Lespedeza cuneata (G. Don) Water Extract on Streptozotocin-induced Type 1 Diabetes and Cytokine-induced Beta-cell Damage

• Kim MS
• Sharma BR
• Rhyu DY

• KMID: 2355142
• DOI: http://doi.org/10.20307/nps.2016.22.3.175

Abstract

The aim of this study was to evaluate the anti-diabetic effects of the water extract of Lespedeza cuneata (LCW) using rat insulinoma (RIN) m5F cells and streptozotocin (STZ)-induced diabetic rats. The effect of LCW on the protection of pancreatic beta cells was assessed using MTT assay, and nitric oxide production was assessed using Griess reagent. STZ-induced diabetic rats were treated with 100 and 400 mg/kg body weight of LCW for 5 weeks. In results, LCW significantly protected cytokine-induced toxicity and NO production, and increased insulin secretion in RINm5F cells. LCW significantly decreased serum blood glucose, thiobarbituric acid reactive substances (TBARS), blood urea nitrogen (BUN) and advanced glycation end products (AGEs) levels, and renal fibronectin expression in STZ-induced diabetic rats. Also, LCW effectively improved BW loss in STZ-induced diabetic rats. Thus, our results suggest that LCW has a beneficial effect on cytokine-induced pancreatic beta cell damage and biomarkers of diabetic complication in hyperglycemic rats.

Keyword

Cytokines; Diabetes; Lesepdeza cuneata; Pancreatic beta cells; Streptozotocin

MeSH Terms

Animals
Biomarkers
Blood Glucose
Blood Urea Nitrogen
Body Weight
Cytokines
Diabetes Complications
Fibronectins
Glycosylation End Products, Advanced
Insulin
Insulin-Secreting Cells
Insulinoma
Lespedeza*
Nitric Oxide
Rats
Streptozocin
Thiobarbituric Acid Reactive Substances
Water*
Biomarkers
Blood Glucose
Cytokines
Fibronectins
Glycosylation End Products, Advanced
Insulin
Nitric Oxide
Streptozocin
Thiobarbituric Acid Reactive Substances
Water

Figure

• 

  Fig. 1. Effects of LCW on IL-1β- and IFN-γ-induced cell viability (A), NO production (B), and insulin secretion (C) in RINm5F cells. RINm5F cells (2 × 106) were pretreated with the indicated concentrations (50 and 100 μg/mL) of LCW for 3 h, followed by stimulation with IL-1β (2 ng/mL) and IFN-γ (100 U/mL) for 48 h. 100 µM TLB was used as a positive control. Each value represents the mean ± SE of three independent experiments. Bars with different letters (a, b, c, and d) are significantly different each other at p < 0.05 in Duncan's multiple comparison tests.

• 

  Fig. 2. Effect of LCW on blood glucose in STZ induced diabetic rats. Values are given as mean ± SE for each group of six animals. Normal, normal rats; control, diabetic control rats, LCW100, treatment with LC extract at dose of 100 mg/kg BW; LCW400, treatment with LC extract at dose of 400 mg/kg BW. Bars with different letters (a, b, and c) are significantly different each other at p < 0.05 in Duncan's multiple comparison tests.

• 

  Fig. 3. Effect of LCW on renal fibronectin expression in STZ-induced diabetic rats. Normal, normal rats; control, diabetic control rats, LCW100, treatment with LC extract at dose of 100 mg/ kg BW; LCW400, treatment with LC extract at dose of 400 mg/ kg BW. Bars with different letters (a and b) are significantly different each other at p < 0.05 in Duncan's multi comparison tests.

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