Nat Prod Sci.  2016 Sep;22(3):162-167. 10.20307/nps.2016.22.3.162.

Simultaneous Determination and Recognition Analysis of Coumarins in Angelica decursiva and Peucedanum praeruptorum by HPLC-DAD

Affiliations
  • 1Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Chungcheongbuk-do 28159, Korea. seongrs@korea.kr

Abstract

Peucedani Radix is the root of Angelica decursiva Franchet et Savatier (=Peucedanum decursivum Maximowicz) or Peucedanum praeruptorum Dunn in several Asian countries. The coumarins contained in Peucedani Radix were quantitatively analyzed using HPLC-DAD to develop a simultaneous determination for the quality control of A. decursiva and P. praeruptorum. For quantitative analysis, four major coumarins contained in these medicinal plants were assessed. Nodakenin (1), nodakenetin (2), praeruptorin A (3), and praeruptorin B (4) were separated with a Phenomenex Luna C18 column (5 µm, 4.6 × 250 mm) under the gradient conditions using distilled water with 0.1% phosphoric acid and acetonitrile with 0.1% phosphoric acid as the mobile phase, at a flow rate of 1.0 ml/min and a detection wavelength of 330 nm. This method was fully validated for linearity, accuracy, precision, recovery, and limit of detection and quantification. As a result, A. decursiva and P. praeruptorum were clearly classified by the quantification of four major coumarins in extracts. Also, the pattern recognition analysis based on HPLC indicates that all of the samples were largely clustered into two groups. Therefore, it is possible to distinguish between A. decursiva and P. praeruptorum and contribute to quality control.

Keyword

Peucedani Radix; Angelica decursiva; Peucedanum praeruptorum; coumarin; HPLC-DAD

MeSH Terms

Angelica*
Asian Continental Ancestry Group
Chromatography, High Pressure Liquid
Coumarins*
Humans
Limit of Detection
Methods
Plants, Medicinal
Quality Control
Water
Coumarins
Water

Figure

  • Fig. 1 Chemical structures of compounds 1 - 4 and I.S.

  • Fig. 2 HPLC chromatograms: (A) Standard mixture, (B) A. decursiva, (C) P. praeruptoruma. aPeak assignment according to Fig. 1.

  • Fig. 3 HClust of A. decursiva (A01 - A09) and P. praeruptorum (P01 - P14).


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