Ewha Med J.  2003 Jun;26(2):137-142. 10.12771/emj.2003.26.2.137.

T Cell Receptor Rearrangement in NKT Cells Differentiated from Cord Blood CD34+ Cells

• Jung YJ
• Woo SY

• KMID: 2352866
• DOI: http://doi.org/10.12771/emj.2003.26.2.137

Abstract

OBJECTIVES
To investigate the time of rearrangement of the TCR gene in the process of NKT cell differentiation from CD34+ human cord blood cells in vitro.
METHODS
We isolated the CD34+ human cord blood cells and induced the differentiation of NKT cells by liquid culture including IL-15, FL and SCF for 30 days. In order to detect the time of TCR gene rearrangement in differentiated NKT cell, we performed PCR for TCR-rearrangement excision circles (TRECs) with isolated DNA.
RESULTS
Signal joint TREC first appeared on day 4 or day 8 and continuously existed until day 30. Between day 9 and day 21, cells showed high output of coding joint TRECs after signal joint rearrangement.
CONCLUSION
In differentiated NKT cells, TCR gene rearrangement started within a week after culture started and mostly occurred in 2 to 3 weeks after culture started.

Keyword

NKT cell; CK34+ cells; TCR gene rearrangement

MeSH Terms

Clinical Coding
DNA
Fetal Blood*
Genes, T-Cell Receptor
Humans
Interleukin-15
Joints
Natural Killer T-Cells*
Polymerase Chain Reaction
Receptors, Antigen, T-Cell*
DNA
Interleukin-15
Receptors, Antigen, T-Cell