Clin Exp Reprod Med.  2016 Sep;43(3):152-156. 10.5653/cerm.2016.43.3.152.

The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers

• Sanmee U
• Piromlertamorn W
• Vutyavanich T

• KMID: 2352848
• DOI: http://doi.org/10.5653/cerm.2016.43.3.152

Abstract

OBJECTIVE
We studied the effect of the site of laser zona opening on the complete hatching of mouse blastocysts and the cell numbers of the completely hatched blastocysts.
METHODS
Mouse blastocysts were randomly allocated to the inner cell mass (ICM) group (zona opening performed at the site of the ICM, n=125), the trophectoderm (TE) group (zona opening performed opposite to the ICM, n=125) and the control group (no zona opening, n=125).
RESULTS
The rate of complete hatching of the blastocysts was not significantly different in the ICM and the TE group (84.8% vs 80.8%, respectively; p=0.402), but was significantly lower in the control group (51.2%, p<0.001). The cell numbers in the completely hatched blastocysts were comparable in the control group, the ICM group, and the TE group (69±19.3, 74±15.7, and 71±16.8, respectively; p=0.680).
CONCLUSION
These findings indicate that the site of laser zona opening did not influence the rate of complete hatching of mouse blastocysts or their cell numbers.

Keyword

Blastocyst; Hatching; Inner cell mass; Lasers; Zona pellucida

MeSH Terms

Animals
Blastocyst*
Cell Count*
Herpes Zoster*
Mice*
Zona Pellucida