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The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers

Sanmee U, Piromlertamorn W, Vutyavanich T

OBJECTIVE: We studied the effect of the site of laser zona opening on the complete hatching of mouse blastocysts and the cell numbers of the completely hatched blastocysts. METHODS: Mouse blastocysts...
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Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women

Pongsuthirak P, Vutyavanich T

OBJECTIVE: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. METHODS: Immature oocytes...
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Rapid freezing versus Cryotop vitrification of mouse two-cell embryos

Inna N, Sanmee U, Saeng-anan U, Piromlertamorn W, Vutyavanich T

OBJECTIVE: To compare our in-house method of embryo freezing with Cryotop vitrification in terms of immediate survival, subsequent cleavage and blastocyst formation, and cell numbers in blastocysts. METHODS: Two-cell mouse embryos...
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The relationship between reactive oxygen species, DNA fragmentation, and sperm parameters in human sperm using simplified sucrose vitrification with or without triple antioxidant supplementation

Juanpanich T, Suttirojpattana T, Parnpai R, Vutyavanich T

Objective: This study examined whether the addition of triple antioxidants (3A)—10 μM acetyl-L-carnitine, 10 μM N-acetyl-L-cysteine, and 5 μM α-lipoic acid—in freezing-thawing medium during human sperm cryopreservation using the sucrose...
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