Clin Exp Reprod Med.  2018 Dec;45(4):189-194. 10.5653/cerm.2018.45.4.189.

Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women

• Pongsuthirak P
• Vutyavanich T

• KMID: 2427415
• DOI: http://doi.org/10.5653/cerm.2018.45.4.189

Abstract

OBJECTIVE
The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women.
METHODS
Immature oocytes were obtained by needle aspiration from 49 pregnant women (group 1) who underwent a cesarean section at term and 77 non-pregnant women (group 2) who underwent a gynecological operation during the same period (8 months). Healthy immature oocytes (530 in group 1 and 539 in group 2) were cultured and assessed for maturation 36 hours later. Mature oocytes were inseminated by intracytoplasmic sperm injection and cultured up to 144 hours.
RESULTS
The percentage of degenerated oocytes was significantly higher (12.1% vs. 6.3%; p < 0.001) in group 1 than in group 2. There was no significant difference in the maturation rate (66.8% vs. 68.1%; p=0.698), fertilization rate (66.7% vs. 67.6%; p=0.857), or the rate of formation of good-quality blastocysts (46.2% vs. 47.2%; p=0.898) in oocytes obtained from pregnant and non-pregnant women.
CONCLUSION
The developmental competence of immature oocytes did not differ between pregnant and non-pregnant women.

Keyword

Competence; Oocyte in vitro maturation; Pregnant women

MeSH Terms

Blastocyst
Cesarean Section
Embryonic Development
Female
Fertilization
Humans*
In Vitro Oocyte Maturation Techniques
Mental Competency*
Needles
Oocytes*
Pregnancy
Pregnant Women
Sperm Injections, Intracytoplasmic