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In vitro maturation: Clinical applications

Lim KS, Chae SJ, Choo CW, Ku YH, Lee HJ, Hur CY, Lim JH, Lee WD

Oocyte in vitro maturation (IVM) is an assisted reproductive technology in which oocytes are retrieved from the antral follicles of unstimulated or minimally stimulated ovaries. IVM of human oocytes has...
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Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation

Yoon J, Juhn KM, Yoon SH, Ko Y, Lim JH

OBJECTIVE: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM),...
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Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels

Kang JT, Kwon DK, Park SJ, Kim SJ, Moon JH, Koo OJ, Jang G, Lee BC

Quercetin is a plant-derived flavonoid found in fruits or vegetables that has antioxidant properties and acts as a free radical scavenger. We investigated the effects of quercetin on porcine oocyte...
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Species-specific Expression of Rpia Transcript in Cumulus-oocyte-complex

Kim YS, Yoon SJ, Kim EY, Lee KA

  • KMID: 2288276
  • Korean J Reprod Med.
  • 2007 Jun;34(2):95-106.
OBJECTIVE: We previously identified differentially expressed genes (DEGs) between germinal vesicle (GV) and metaphase II (MII) mouse oocyte.(1) The present study was accomplished as a preliminary study to elucidate the...
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Cryopreservation of in vitro matured oocytes after ex vivo oocyte retrieval from gynecologic cancer patients undergoing radical surgery

Park CW, Lee SH, Yang KM, Lee IH, Lim KT, Lee KH, Kim TJ

OBJECTIVE: The aim of this study was to report a case series of in vitro matured (IVM) oocyte freezing in gynecologic cancer patients undergoing radical surgery under time constraints as...
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Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women

Pongsuthirak P, Vutyavanich T

OBJECTIVE: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. METHODS: Immature oocytes...
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Simultaneous Detection of Seven Phosphoproteins in a Single Lysate Sample during Oocyte Maturation Process

Yoon SJ, Kim YS, Kim KH, Yoon TK, Lee WS, Lee KA

  • KMID: 1473569
  • Korean J Reprod Med.
  • 2009 Sep;36(3):187-198.
OBJECTIVE: Phosphorylation and dephosphorylation of proteins are important in regulating cellular signaling pathways. Bead-based multiplex phosphorylation assay was conducted to detect the phosphorylation of seven proteins to maximize the information...
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Expression of interferon regulatory factor-1 in the mouse cumulus-oocyte complex is negatively related with oocyte maturation

Kim YS, Kim EY, Moon J, Yoon TK, Lee WS, Lee KA

OBJECTIVE: We found previously that interferon regulatory factor (Irf)-1 is a germinal vesicle (GV)-selective gene that highly expressed in GV as compared to metaphase II oocytes. To our knowledge, the...
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The potential role of granulosa cells in the maturation rate of immature human oocytes and embryo development: A co-culture study

Jahromi BN, Mosallanezhad Z, Matloob N, Davari M, Ghobadifar MA

OBJECTIVE: In order to increase the number of mature oocytes usable for intracytoplasmic sperm injection (ICSI), we aimed to investigate the effect of co-culturing granulosa cells (GCs) on human oocyte...
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Rapamycin treatment during in vitro maturation of oocytes improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs

Lee J, Park JI, Yun JI, Lee Y, Yong H, Lee ST, Park CK, Hyun SH, Lee GS, Lee E

This study was conducted to investigate the effects of rapamycin treatment during in vitro maturation (IVM) on oocyte maturation and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear...
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Development of in vitro produced porcine embryos according to serum types as macromolecule

Son J, Malaweera , Lee E, Shin S, Cho J

This study was conducted to establish an in vitro maturation (IVM) system by selection of efficient porcine serum during porcine in vitro production. To investigate the efficient porcine serum (PS),...
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Application of serum anti-Müllerian hormone levels in selecting patients with polycystic ovary syndrome for in vitro maturation treatment

Seok HH, Song H, Lyu SW, Kim YS, Lee DR, Lee WS, Yoon TK

OBJECTIVE: The purpose of this study was to identify useful clinical factors for the identification of patients with polycystic ovary syndrome (PCOS) who would benefit from in vitro maturation (IVM)...
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