Korean J Parasitol.  2016 Jun;54(3):375-380. 10.3347/kjp.2016.54.3.375.

Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection

Affiliations
  • 1National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, Key Laboratory of Parasite and Vector Biology, National Health and Family Planning Commission, Shanghai 200025, PR
  • 2Shenzhen Center for Diseases Control and Prevention, Shenzhen, Guangdong Province 518055, PR China. renlizhang@tom.com
  • 3Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200438, PR China.

Abstract

Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.

Keyword

Angiostrongylus cantonensis; lateral flow immunoassay; monoclonal antibody; antigen detection

MeSH Terms

Adult
Angiostrongylus cantonensis*
Angiostrongylus*
Antibodies, Monoclonal
Diagnosis
Enzyme-Linked Immunosorbent Assay
Humans*
Immunoassay*
Limit of Detection
Methods
Sensitivity and Specificity
Serologic Tests
Antibodies, Monoclonal
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