Korean J Parasitol.  2016 Jun;54(3):375-380. 10.3347/kjp.2016.54.3.375.

Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection

• Chen MX
• Chen JX
• Chen SH
• Huang DN
• Ai L
• Zhang RL

• KMID: 2348281
• DOI: http://doi.org/10.3347/kjp.2016.54.3.375

Abstract

Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.

Keyword

Angiostrongylus cantonensis; lateral flow immunoassay; monoclonal antibody; antigen detection

MeSH Terms

Adult
Angiostrongylus cantonensis*
Angiostrongylus*
Antibodies, Monoclonal
Diagnosis
Enzyme-Linked Immunosorbent Assay
Humans*
Immunoassay*
Limit of Detection
Methods
Sensitivity and Specificity
Serologic Tests
Antibodies, Monoclonal