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Cortisol Measurements in Cushing's Syndrome: Immunoassay or Mass Spectrometry?

Casals G, Hanzu FA

Determination of cortisol levels in the urine (24 hours urine free cortisol), saliva (late-night), or serum (total cortisol after dexamethasone suppression) is recommended to screen for Cushing's syndrome (CS). This...
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Comparison of Biotin Interference in Second- and Third-Generation Roche Free Thyroxine Immunoassays

Choi J, Yun SG

No abstract available.
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Distinct Inflammation Biomarkers in Healthy Individuals and Patients with Schizophrenia: A Reliability Testing of Multiplex Cytokine Immunoassay by Bland-Altman Analysis

Yeh TC, Chu HT, Tsai CK, Chang HA, Yang FC, Huang SY, Liang CS

OBJECTIVE: Since the inflammatory process has been implicated in the pathophysiology of psychiatric disorder, an important issue emerging is to assess the test-retest reliability of cytokine measurement in healthy individuals...
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Multicenter Comparison of Four Automated Immunoassay Analyzers for Prostate Specific Antigen

Park DJ, Kim Y, Lee HK, Lee J, Han K, Kwon HJ

No abstract available.
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Comparison of Six Automated Immunoassays With Isotope-Diluted Liquid Chromatography-Tandem Mass Spectrometry for Total Thyroxine Measurement

Yu S, Zhou W, Cheng X, Meng Q, Li H, Hou L, Lu J, Xie S, Cheng Q, Zhang C, Qiu L

BACKGROUND: Accurate serum total thyroxine (TT4) measurement is important for thyroid disorder diagnosis and management. We compared the performance of six automated immunoassays with that of isotope-diluted liquid chromatography-tandem mass...
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The novel latex agglutination turbidimetric immunoassay system for simultaneous measurements of calprotectin and hemoglobin in feces

Hiraoka S, Takashima , Inokuchi T, Nakarai A, Takahara M, Harada K, Seki Y, Watanabe K, Kato J, Okada H

BACKGROUND/AIMS: Fecal calprotectin (Fcal) as well as the fecal immunochemical test (FIT) are useful biomarkers for detecting activity and mucosal healing in inflammatory bowel diseases. Here, we report the performance...
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Optimization of ultra-fast convection polymerase chain reaction conditions for pathogen detection with nucleic acid lateral flow immunoassay

Kim TH, Hwang HJ, Kim JH

Recently, the importance of on-site detection of pathogens has drawn attention in the field of molecular diagnostics. Unlike in a laboratory environment, on-site detection of pathogens is performed under limited...
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Performance Evaluation of the Automated Fluorescent Immunoassay System Rotavirus Assay in Clinical Samples

Kim JS, Lee SK, Ko DH, Hyun J, Kim HS

BACKGROUND: The Automated Fluorescent Immunoassay System (AFIAS) rotavirus assay (Boditech Med Inc., Chuncheon, Korea) is a new rapid antigen test for rotavirus detection. We evaluated the performance of this assay...
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Analysis of Reentry Test for the Donors Showing Reactivity or Grey Zone in a HBV Surface Antigen Assay by a Chemiluminescent Immunoassay

Shin S, Kang J, Lee KR, Shin GS, Kang JW, Seo YI, Min H

BACKGROUND: If donors who were deferred due to the reactivity or grey zone in HBV surface antigen (HBsAg) assay want to donate blood again, they need to pass reentry tests....
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Development of a Rapid Automated Fluorescent Lateral Flow Immunoassay to Detect Hepatitis B Surface Antigen (HBsAg), Antibody to HBsAg, and Antibody to Hepatitis C

Ryu JH, Kwon M, Moon JD, Hwang MW, Lee JM, Park KH, Yun SJ, Bae HJ, Choi A, Lee H, Jung B, Jeong J, Han K, Kim Y, Oh EJ

BACKGROUND: Accurate, rapid, and cost-effective screening tests for hepatitis B virus (HBV) and hepatitis C virus (HCV) infection may be useful in laboratories that cannot afford automated chemiluminescent immunoassays (CLIAs)....
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The Role of the Signal-to-Cutoff Ratio in Automated Anti-HCV Chemiluminescent Immunoassays by Referring to the Nucleic Acid Amplification Test and the Recombinant Immunoblot Assay

Choi MS, Lee K, Hong YJ, Song EY, Kim DS, Song J

BACKGROUND: Following discontinuation of the recombinant immunoblot assay (RIBA), the only available supplementary test for the detection of hepatitis C virus (HCV) is the nucleic acid amplification test (NAAT). However,...
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Comparison of Four Automated Carcinoembryonic Antigen Immunoassays: ADVIA Centaur XP, ARCHITECT I2000sr, Elecsys E170, and Unicel Dxi800

Park J, Lee S, Kim Y, Choi A, Lee H, Lim J, Kim Y, Han K, Oh EJ

BACKGROUND: Carcinoembryonic antigen (CEA) is one of the tumor markers available for evaluating disease progression status after initial therapy and monitoring subsequent treatment modalities in colorectal, gastrointestinal, lung, and breast...
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Multi-center Performance Evaluations of Tacrolimus and Cyclosporine Electrochemiluminescence Immunoassays in the Asia-Pacific Region

Qin X, Rui J, Xia Y, Mu H, Song SH, Raja Aziddin RE, Miles G, Sun Y, Chun S

BACKGROUND: The immunosuppressant drugs (ISDs), tacrolimus and cyclosporine, are vital for solid organ transplant patients to prevent rejection. However, toxicity is a concern, and absorption is highly variable across patients;...
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Comparison of Singleplex Specific IgE Detection Immunoassays: ImmunoCAP Phadia 250 and Immulite 2000 3gAllergy

Park KH, Lee J, Sim DW, Lee SC

BACKGROUND: The identification of clinically meaningful specific immunoglobulin E (sIgE) is important for the diagnosis and management of allergic diseases. Various in vitro sIgE detection methods are available worldwide. Depending...
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Assessment of the Precision and Functional Sensitivity of Two Thyroglobulin Assays: Comparison of the Second-Generation Roche Electrochemiluminescent Immunoassay and BRAHAMS Radioimmunoassay

Kwon A, Lee EH, Lee YK, Kang HJ

BACKGROUND: Thyroglobulin (Tg) is the primary biochemical marker used to monitor patients with differentiated thyroid cancer (DTC) for residual or recurrent disease after total thyroidectomy, as only normal or well-differentiated...
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Annual Report on the External Quality Assessment Scheme for Immunoassay Tests in Korea (2015)

Kim HS, Kim YL, Shim JE, Immunoassay Subcommittee, Korean Association of External Quality Assessment Services

Two trials of external quality assessment were performed in 2015, with 13 test items grouped into four test categories. The first trial materials were sent on May 19, 2015 and...
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Falsely Elevated Tacrolimus Concentrations Using Chemiluminescence Microparticle Immunoassay in Kidney Transplant Patient

Yang D, Song SA, Jun KR, Rim H, Lee W

Tacrolimus is one of the effective immunosuppressive drugs used after an organ transplant procedure. However, due to its narrow therapeutic range, its usefulness in preventing transplant rejection and minimizing nephrotoxicity...
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Comparison of Thyroglobulin Measurements Using Three Different Immunoassay Kits: A BRAMHS Tg-Plus RIA Kit, a BRAMHS hTg Sensitive Kryptor Kit, and a Beckman Coulter ACCESS Immunoassay Kit

Kim M, Jeon MJ, Kim WG, Lee JJ, Ryu JS, Cho EJ, Ko DH, Lee W, Chun S, Min WK, Kim TY, Shong YK, Kim WB

BACKGROUND: Second-generation thyroglobulin immunometric assays (Tg-IMAs) have been developed with improved sensitivity. Our aim was to compare the diagnostic value of Tg-IMA measurements using a Kryptor (BRAHMS AG) kit (Tg-K)...
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Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection

Chen MX, Chen JX, Chen SH, Huang DN, Ai L, Zhang RL

Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and...
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Annual Report on External Quality Assessment Scheme for Immunoassay Tests in Korea (2014)

Kim HS, Kim YL, Immunoassay Subcommittee, Korean Association of External Quality Assessment Service

In 2014, two external quality assessment trials were performed on 13 test items grouped in four categories. The laboratories procured the materials for the first and second trials on 13...
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