J Korean Neurol Assoc.
2000 Nov;18(6):728-734.
Effects of the Immunoglobulins of Amyotrophic Lateral Sclerosis on Intracellular Calcium in PC12 Cells
- Affiliations
-
- 1Department of Neurology, College of Medicine, Seoul National University.
- 2Department of Physiology, College of Medicine, Seoul National University.
- 3Department of Neurology, Seoul Paik Hospital.
- 4Department of Physiology, College of Medicine, Dankook University.
Abstract
-
BACKGROUND: The motor neuronal death in amyotrophic lateral sclerosis (ALS) arise from several mechanisms including excitotoxicity, oxidative stress, immunologic or calcium dysregulation. Though the immunoglobulin mediated neuronal death has been implicated through changes of calcium influx, the direct evidence showing the increase of intracellular calcium level is rare. The purpose of our study is to directly measure the calcium in cultured neurons treated with immunoglobulins from ALS patients to further understand the role of calcium in motor neuron degeneration.
METHODS
Immunoglobins purified from serum in patients with ALS (n=8) and in control (n=8) were incubated for 24 hours in PC12 cell line. The intracellular calcium level was measured by fluorometry using calcium chelating dye, fura-2. High concentration of potassium chloride were used to evoke calcium influx, and L-type calcium channel current were measured using nicardipine, the L-type specific calcium channel blocker.
RESULTS
Basal level of intracellular calcium was higher in the cells treated with immunoglobulins from ALS. However, the calcium increase evoked by 30 mM or 140 mM potassium chloride were lower in ALS than in control. The calcium increase through L-type calcium channel in ALS group was not significantly different from control. Conclusions ; Considering the proposed role of the calcium in neuronal degeneration, present data suggest increased intracellular calcium is important in neurodegenerative mechanism caused by ALS immunoglobulins.
MeSH Terms
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Amyotrophic Lateral Sclerosis Associated With CADASIL
- Syndrome of Progressive Bulbar Palsy in Amyotrophic Lateral Sclerosis: A Case Report
- Apraxia of Eyelid Closure and Motor Impersistence of Eyelid in a Patient with Amyotrophic Lateral Sclerosis
- The Effect of Calcium Modulators on Motoneuron Cells Which Express Mutated Cu / Zn Superoxide Dismutase
- Psychosocial Responses and Quality of Life among Amyotrophic Lateral Sclerosis Patients and Their Caregivers
