J Korean Ophthalmol Soc.  2002 Aug;43(8):1503-1509.

Effects of Corneal Neovascularization on TNF-alpha and TGF-beta 2 mRNA Expression in Endotoxin Induced Uveitis

• Choi SJ
• Kim JW
• Kim JW
• Park JY

Abstract

PURPOSE: We tried to evaluate the expression of TNF-alpha and TGF-beta 2 mRNA during the course of the endotoxin induced uveitis (EIU) in rat after inducing neovascularization on cornea.
METHODS
Reverse transcription followed by polymerase chain reaction amplification was used to determine relative mRNA level in two ocular tissues (iris-ciliary body, cornea) at 0, 1, 3, 6, 12, 48 hours after subcutaneous injection of 200 ug of Escherichia coli endotoxin. RESULT: Rat with corneal neovascularization showed somewhat different pattern of expression. TGF-beta 2 mRNA expression was showed some fluctuations in cornea. TGF-beta 2 mRNA expression in cornea was decreased within 3 hours after endotoxin treatment and increased gradually after 6 hours until 48 hours.
CONCLUSION
TGF-beta2 might be one of the important cytokines in EIU, especially animals with neovascularization on cornea.

Keyword

Endotoxin induced uveitis; Endotoxin; TNF-alpha; TGF-beta2

MeSH Terms

Animals
Cornea
Corneal Neovascularization*
Cytokines
Escherichia coli
Injections, Subcutaneous
Polymerase Chain Reaction
Rats
Reverse Transcription
RNA, Messenger*
Transforming Growth Factor beta*
Transforming Growth Factor beta2
Tumor Necrosis Factor-alpha*
Uveitis*
Cytokines
RNA, Messenger
Transforming Growth Factor beta
Transforming Growth Factor beta2
Tumor Necrosis Factor-alpha