J Korean Soc Biol Psychiatry.  1999 Jul;6(1):67-73.

Screening of Differentially Expressed Genes between PC12 Cells and A123.7 Cells

• Baik SY
• Chai YG
• Yang BH

Abstract

The cAMP-dependent protein kinase(PKA) is an intracellular enzyme with serine-threonine kinase activity that plays a key role in cell growth, differentiation, and apoptosis in eukaryotes. In order to understand the PKA signal transduction pathway regulating cell life cycle and identify its role, we focused on the characterization of up-/down-regulated genes by PKA using the differential display polymerase chain reaction. Seven differentially expressed sequence tags(DEST) have been obtained. Among these DESTs, 2DESTs were homologous to the sequence of genes from BLAST search result. KC1-5 DEST that was up-regulated in A123.7 cells was highly corresponded to mouse apoptosis-related gene(MA-3) or mouse mRNA for topoisomerase inhibitor suppressed(TIS). MA-3 was induced in various types of apoptosis, specially in NGF-deprived apoptotic PC12 cells, TIS was down-regulated in the RVC lymphoma cells incubated with topoisomerase inhibitor that induces DNA strand breakages. PG1-1DEST that was highly expressed in PC12 cells was corresponded to transposon Tn103'-end. Tnansposon Tn10 was up-regulated in differentiated myeloblastic ML-1 cells by 12-O-tetradecanoylphorbol-13-acetate. This study illuminates that MA-3/TIS was down-regulated by PKA activity, and transposon Tn10 was up-regulated by it.

MeSH Terms

Animals
Apoptosis
DNA
Eukaryota
Granulocyte Precursor Cells
Life Cycle Stages
Lymphoma
Mass Screening*
Mice
PC12 Cells*
Polymerase Chain Reaction
Protein-Serine-Threonine Kinases
RNA, Messenger
Signal Transduction
DNA
Protein-Serine-Threonine Kinases
RNA, Messenger