J Korean Continence Soc.  2009 Dec;13(2):142-151.

The Beneficial Effect of Korean Red Ginseng on Rat Bladder Contractility and Oxidant Damage Following Ischemia/Reperfusion

• Shin JH
• Oh TH
• Yang SW
• Youn CS
• Kim YH
• Hwang EG
• Kim YW
• Yuk SM
• Lim JS
• Song KH
• Sul CK
• Na YG

Abstract

PURPOSE
N-acetylcysteine (NAC) is a potent antioxidant, and a free radical scavenger. We investigated the possible effects of NAC after ischemia/reperfusion (I/R) of rat bladder.
MATERIALS AND METHODS
I/R injury was induced by abdominal aorta clamping and ischemia for 60minutes, followed by 120minutes reperfusion. Twenty rats were divided into four groups: sham operation + saline group (S+S), sham operation + NAC group (S+NAC), I/R + saline group (I/R+S), I/R + NAC group (I/R+NAC). Blood levels of reactive oxygen species (ROS) were determined using the free oxygen radical tests (FORT). Superoxide generation was measured based on lucigenin-enhanced chemiluminescence. The level of malondialdehyde (MDA) was analyzed in order to measure lipid peroxidation.
RESULTS
In I/R+S group, the isometric contractile responses to carbachol were significant lower than other groups and were reversed by the pretreatment with NAC. The level of FORT and MDA showed a marked increase in I/R+S group compared with S+S group. NADPH-stimulated superoxide production was also significantly increased. I/R+NAC decreased these parameters compared with I/R+S group.
CONCLUSION
Our results suggest that treatment with NAC reversed the low contractile responses of rat bladder and prevented oxidative stress following I/R.

Keyword

Bladder; Ischemia; Reperfusion; N-acetylcysteine

MeSH Terms

Acetylcysteine
Animals
Aorta, Abdominal
Carbachol
Constriction
Ischemia
Lipid Peroxidation
Luminescence
Malondialdehyde
Oxidative Stress
Oxygen
Panax*
Rats*
Reactive Oxygen Species
Reperfusion
Superoxides
Urinary Bladder*
Acetylcysteine
Carbachol
Malondialdehyde
Oxygen
Reactive Oxygen Species
Superoxides