J Korean Cancer Assoc.  1998 Jun;30(3):527-535.

The p16INK4A Expression in Stomach Cancer , Colon Cancer and Hepatoma Cell Lines

• Choi SJ
• Kim SK
• Kim SJ
• Koh CM
• Park YS

Abstract

PURPOSE
The p16(INK4A) gene encodes a specific inhibitor of cell cycle progression. In recent years, genetic deletion and altered expression of p16(INK4A) gene were frequently showed in many human cancers. So, the p16(INK4A) gene is considered as tumor suppressor gene. However, there has been a few data for the p16(INK4A) in gastric cancer, colon cancer, and hepatoma.So.we investigated the genetic deldtion and altered expression of p16(INK4A) in gastric cancer, colon cancer and hepatoma cell lines.
MATERIALS AND METHODS
The homozygous deletion of p16(INK4A) was examined by using PCR and the protein expression of p16(INK4A) by using Western blotting in cancer cell lines established from Korean patients: stomach cancer, colon cancer and hepatoma cell lines.
RESULTS
Homozygous deletion of p16(INK4A) was detected only 1 stomach cancer cell line out of 13 cell lines examined. The p16(INK4A) was detected in 3 of 13 cancer cell line. These results showed the low frequency of p16(INK4A) homozygous deletion and high frequency of p16(INK4A) expression alteration in stomach cancer, colon cancer and hepatoma cell lines.
CONCLUSION
In this study, it may be suggested that the altered pl6(INK4A) expression as well as p16(INK4A) gene deletion play important role in oncogenesis. Further studies to determine the mechanism of p16(INK4A) gene inactivation are expected.

Keyword

Cancer cell line; p16 (INK4A) homozygous deletion; p16(INK4A) expression

MeSH Terms

Blotting, Western
Carcinogenesis
Carcinoma, Hepatocellular*
Cell Cycle
Cell Line*
Colon*
Colonic Neoplasms*
Cyclin-Dependent Kinase Inhibitor p16
Gene Deletion
Gene Silencing
Genes, Tumor Suppressor
Humans
Polymerase Chain Reaction
Stomach Neoplasms*
Stomach*
Cyclin-Dependent Kinase Inhibitor p16